Toward the use of nanopore RNA sequencing technologies in the clinic: challenges and opportunities
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- dc.contributor.author Katopodi, Xanthi-Lida
- dc.contributor.author Begik, Oguzhan
- dc.contributor.author Novoa, Eva Maria
- dc.date.accessioned 2025-05-15T06:55:51Z
- dc.date.available 2025-05-15T06:55:51Z
- dc.date.issued 2025
- dc.description.abstract RNA molecules have garnered increased attention as potential clinical biomarkers in recent years. While short-read sequencing and quantitative polymerase chain reaction have been the primary methods for quantifying RNA abundance, they typically fail to capture critical post-transcriptional regulatory elements, such as RNA modifications, which are often dysregulated in disease contexts. A promising cutting-edge technique sequencing method that addresses this gap is direct RNA sequencing, offered by Oxford Nanopore Technologies, which can simultaneously capture both RNA abundance and modification information. The rapid advancements in this platform, along with growing evidence of dysregulated RNA species in biofluids, presents a compelling clinical opportunity. In this review, we discuss the challenges and the emerging opportunities for the adoption of nanopore RNA sequencing technologies in the clinic, highlighting their potential to revolutionize personalized medicine and disease monitoring.
- dc.format.mimetype application/pdf
- dc.identifier.citation Katopodi XL, Begik O, Novoa EM. Toward the use of nanopore RNA sequencing technologies in the clinic: challenges and opportunities. Nucleic Acids Res. 2025 Feb 27;53(5):gkaf128. DOI: 10.1093/nar/gkaf128
- dc.identifier.doi http://dx.doi.org/10.1093/nar/gkaf128
- dc.identifier.issn 0305-1048
- dc.identifier.uri http://hdl.handle.net/10230/70400
- dc.language.iso eng
- dc.publisher Oxford University Press
- dc.relation.ispartof Nucleic Acids Res. 2025 Feb 27;53(5):gkaf128
- dc.rights © The Author(s) 2025. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
- dc.subject.other RNA
- dc.title Toward the use of nanopore RNA sequencing technologies in the clinic: challenges and opportunities
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion