Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Ferro, P.
- dc.contributor.author Krotov, G.
- dc.contributor.author Zvereva, I.
- dc.contributor.author Pérez Mañá, Clara
- dc.contributor.author Mateus Rodriguez, Julian Andrés
- dc.contributor.author Segura, Joan
- dc.date.accessioned 2020-12-15T08:05:21Z
- dc.date.available 2020-12-15T08:05:21Z
- dc.date.issued 2017
- dc.description.abstract The use of small peptides for medical purposes has gained great importance in the last decade. Among these molecules are the growth hormone releasing peptides (GHRPs). These substances are highly sought by cheating athletes because of their ergogenic effects. Thus, GHRPs are listed in the World Antidoping Agency Prohibited List and several methods have been developed for their detection in urine samples. Serum is another useful matrix frequently used for anti-doping control. An experimental adaptation of a competitive methodology for GHRPs wide-group detection, previously developed for urine, is reported here using a serum matrix instead. The modifications have been mainly addressed the need to remove endogenous ghrelin (always present in serum) to avoid interference in the competitive binding assay. The modified test was first evaluated using serum spiked with different pure GHRPs (i.e., GHRP-2, GHRP-6 and Hexarelin), then using serum samples from pilot studies in which healthy subjects were treated intravenously with GHRP-2 and intranasally with GHRP-2 and GHRP-6. The results showed the reliability of the modified methodology for the detection of GHRPs in a serum matrix
- dc.format.mimetype application/pdf
- dc.identifier.citation Ferro P, Krotov G, Zvereva I, Pérez-Mana, Mateus JA, et al. Detection of Growth Hormone Releasing Peptides in Serum by a Competitive Receptor Binding Assay. J Chromatogr Sep Tech 2017. 8: 351. DOI: 10.4172/2157-7064.1000351
- dc.identifier.doi http://dx.doi.org/10.4172/2157-7064.1000351
- dc.identifier.issn 2157-7064
- dc.identifier.uri http://hdl.handle.net/10230/46039
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Journal of Chromatography and Separation Techniques. 2017;8:351
- dc.rights Copyright: © 2017 Ferro P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.keyword Growth hormone secretagogue
- dc.subject.keyword Ghrelin
- dc.subject.keyword Radiocompetition assay
- dc.subject.keyword Doping substances
- dc.subject.keyword Doping abuse
- dc.title Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion