Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells
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- dc.contributor.author Chen, Tzu-Chun
- dc.contributor.author Talló Parra, Marc, 1992-
- dc.contributor.author Cao, Qian M.
- dc.contributor.author Kadener, Sebastian
- dc.contributor.author Böttcher, René
- dc.contributor.author Pérez Vilaró, Gemma, 1985-
- dc.contributor.author Boonchuen, Pakpoom
- dc.contributor.author Somboonwiwat, Kunlaya
- dc.contributor.author Díez Antón, Juana, 1962-
- dc.contributor.author Sarnow, Peter
- dc.date.accessioned 2021-03-10T11:08:50Z
- dc.date.available 2021-03-10T11:08:50Z
- dc.date.issued 2020
- dc.description.abstract Viruses subvert macromolecular pathways in infected host cells to aid in viral gene amplification or to counteract innate immune responses. Roles for host-encoded, noncoding RNAs, including microRNAs, have been found to provide pro- and anti-viral functions. Recently, circular RNAs (circRNAs), that are generated by a nuclear back-splicing mechanism of pre-mRNAs, have been implicated to have roles in DNA virus-infected cells. This study examines the circular RNA landscape in uninfected and hepatitis C virus (HCV)-infected liver cells. Results showed that the abundances of distinct classes of circRNAs were up-regulated or down-regulated in infected cells. Identified circRNAs displayed pro-viral effects. One particular up-regulated circRNA, circPSD3, displayed a very pronounced effect on viral RNA abundances in both hepatitis C virus- and Dengue virus-infected cells. Though circPSD3 has been shown to bind factor eIF4A3 that modulates the cellular nonsense-mediated decay (NMD) pathway, circPSD3 regulates RNA amplification in a pro-viral manner at a post-translational step, while eIF4A3 exhibits the anti-viral property of the NMD pathway. Findings from the global analyses of the circular RNA landscape argue that pro-, and likely, anti-viral functions are executed by circRNAs that modulate viral gene expression as well as host pathways. Because of their long half-lives, circRNAs likely play hitherto unknown, important roles in viral pathogenesis.
- dc.description.sponsorship This study was funded by the National Institutes of Health (https://www.nih.gov) (R01AI06900011) and NIH grants R01GM122406 and R01AG057700 to S.K. Funds for J.D. were received from the Spanish Ministry of Economy and Competitiveness (AEI/ MINECO/FEDER,UE) through grants BFU2016–80039-R and Unidad de Excelencia Maria de Maeztu funded by the MINECO (ref: MDM-2014–0370), and by a 2017 SGR 909 grant by the Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement of the Generalitat de Catalunya
- dc.format.mimetype application/pdf
- dc.identifier.citation Chen T, Tallo-Parra M, Cao QM, Kadener S, Böttcher R, Pérez-Vilaró G et al. Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells. PLoS Pathog. 2020 Aug 7;16(8):e1008346. DOI: 10.1371/journal.ppat.1008346
- dc.identifier.doi http://dx.doi.org/10.1371/journal.ppat.1008346
- dc.identifier.issn 1553-7366
- dc.identifier.uri http://hdl.handle.net/10230/46718
- dc.language.iso eng
- dc.publisher Public Library of Science (PLoS)
- dc.relation.ispartof PLOS Pathogens. 2020 Aug 7;16(8):e1008346
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2016–80039-R
- dc.rights © 2020 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.other Virus de l'hepatitis C
- dc.subject.other Genètica
- dc.subject.other Fetge
- dc.title Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion