Diagnostic value of genotypic analysis in primary cutaneous lymphomas using standardized BIOMED-2 polymerase chain reaction protocols: experience in daily clinical practice

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• dc.contributor.author López-Aventín, Daniel
• dc.contributor.author Gallardo Hernández, Fernando
• dc.contributor.author Colomo Saperas, Luis Alberto
• dc.contributor.author Moragón, Ester
• dc.contributor.author Vela Ortíz, María del Carmen
• dc.contributor.author Duran Jordà, Xavier, 1974-
• dc.contributor.author Bellosillo Paricio, Beatriz
• dc.contributor.author Pujol Vallverdú, Ramon Maria
• dc.date.accessioned 2021-08-26T06:54:29Z
• dc.date.available 2021-08-26T06:54:29Z
• dc.date.issued 2021
• dc.description.abstract BIOMED-2 Concerted Action BMH4-CT98-3936 (BIOMED-2) PCR protocols are an important diagnostic tool in the evaluation of cutaneous lymphomas. The aim of this study was to assess the diagnostic value of the genotyping results obtained by these techniques in daily clinical practice. A total of 360 paraffin-embedded skin samples were retrospectively reviewed from 114 cutaneous T-cell lymphomas and 35 cutaneous B-cell lym-phomas. A total of 249 biopsies from 180 patients with benign lymphoid infiltrates served as controls. T-cell receptor and immunoglobulin gene rearrangements were assessed using the BIOMED-2 method. A combined T-cell receptor gamma and beta assay approach reliably distinguished cutaneous T-cell lymphomas from benign skin T-cell infiltrates (sensitivity 89.4%; specificity 81.5%). Analysis of complete immunoglobulin heavy chain rearrangements also differentiated cutaneous B-cell lymphomas from benign B-cell infiltrates (sensitivity 85.7%; specificity 82.4%). In conclusion, the full BIOMED-2 protocol is a useful aid combined with clinical, histological and immunophenotypical findings for assessment of lymphoid clonality in skin lymphoid proliferations.
• dc.format.mimetype application/pdf
• dc.identifier.citation López Aventín D, Gallardo F, Colomo L, Moragón E, Vela MC, Duran Jordà X, Bellosillo B, Pujol RM. Diagnostic value of genotypic analysis in primary cutaneous lymphomas using standardized BIOMED-2 polymerase chain reaction protocols: experience in daily clinical practice. Acta Derm Venereol. 2021;101(5):adv00460. DOI: 10.2340/00015555-3828
• dc.identifier.doi http://dx.doi.org/10.2340/00015555-3828
• dc.identifier.issn 0001-5555
• dc.identifier.uri http://hdl.handle.net/10230/48332
• dc.language.iso eng
• dc.publisher Society for Publication of Acta Dermato-Venereologica
• dc.relation.ispartof Acta Derm Venereol. 2021;101(5):adv00460
• dc.rights This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
• dc.subject.keyword T-cell receptor genes
• dc.subject.keyword Clonality
• dc.subject.keyword Cutaneous B-cell lymphoma
• dc.subject.keyword Cutaneous T-cell lymphoma
• dc.subject.keyword Immunoglobulin genes
• dc.subject.keyword Multiplex polymerase chain reaction
• dc.subject.keyword BIOMED-2
• dc.title Diagnostic value of genotypic analysis in primary cutaneous lymphomas using standardized BIOMED-2 polymerase chain reaction protocols: experience in daily clinical practice
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion