Retention of paternal DNA methylome in the developing zebrafish germline

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• dc.contributor.author Skvortsova, Ksenia
• dc.contributor.author Tarbashevich, Katsiaryna
• dc.contributor.author Stehling, Martin
• dc.contributor.author Lister, Ryan
• dc.contributor.author Irimia Martínez, Manuel
• dc.contributor.author Raz, Erez
• dc.contributor.author Bogdanovic, Ozren
• dc.date.accessioned 2019-10-01T08:06:04Z
• dc.date.available 2019-10-01T08:06:04Z
• dc.date.issued 2019
• dc.description.abstract Two waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish PGCs at four developmental stages and identify retention of paternal epigenetic memory, in stark contrast to the findings in mammals. Gene expression profiling of zebrafish PGCs at the same developmental stages revealed that the embryonic germline is defined by a small number of markers that display strong developmental stage-specificity and that are independent of DNA methylation-mediated regulation. We identified promoters that are specifically targeted by DNA methylation in somatic and germline tissues during vertebrate embryogenesis and that are frequently misregulated in human cancers. Together, these detailed methylome and transcriptome maps of the zebrafish germline provide insight into vertebrate DNA methylation reprogramming and enhance our understanding of the relationships between germline fate acquisition and oncogenesis.
• dc.format.mimetype application/pdf
• dc.identifier.citation Skvortsova K, Tarbashevich K, Stehling M, Lister R, Irimia M, Raz E et al. Retention of paternal DNA methylome in the developing zebrafish germline. Nat Commun. 2019;10(1):3054. DOI: 10.1038/s41467-019-10895-6
• dc.identifier.doi http://dx.doi.org/10.1038/s41467-019-10895-6
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/42360
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Nature Communications. 2019;10(1):3054
• dc.rights © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword DNA methylation
• dc.subject.keyword Epigenetic memory
• dc.subject.keyword Epigenetics
• dc.subject.keyword Epigenomics
• dc.subject.keyword Germline development
• dc.title Retention of paternal DNA methylome in the developing zebrafish germline
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion