Modeling of the HIV-1 life cycle in productively infected cells to predict novel therapeutic targets

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• dc.contributor.author Shcherbatova, Olga
• dc.contributor.author Grebennikov, Dmitry
• dc.contributor.author Sazonov, Igor
• dc.contributor.author Meyerhans, Andreas
• dc.contributor.author Bocharov, Gennady A.
• dc.date.accessioned 2020-05-06T07:10:40Z
• dc.date.available 2020-05-06T07:10:40Z
• dc.date.issued 2020
• dc.description.abstract There are many studies that model the within-host population dynamics of Human Immunodeficiency Virus Type 1 (HIV-1) infection. However, the within-infected-cell replication of HIV-1 remains to be not comprehensively addressed. There exist rather few quantitative models describing the regulation of the HIV-1 life cycle at the intracellular level. In treatment of HIV-1 infection, there remain issues related to side-effects and drug-resistance that require further search "...for new and better drugs, ideally targeting multiple independent steps in the HIV-1 replication cycle" (as highlighted recently by Teldury et al., The Future of HIV-1 Therapeutics, 2015). High-resolution mathematical models of HIV-1 growth in infected cells provide an additional analytical tool in identifying novel drug targets. We formulate a high-dimensional model describing the biochemical reactions underlying the replication of HIV-1 in target cells. The model considers a nonlinear regulation of the transcription of HIV-1 mediated by Tat and the Rev-dependent transport of fully spliced and singly spliced transcripts from the nucleus to the cytoplasm. The model is calibrated using available information on the kinetics of various stages of HIV-1 replication. The sensitivity analysis of the model is performed to rank the biochemical processes of HIV-1 replication with respect to their impact on the net production of virions by one actively infected cell. The ranking of the sensitivity factors provides a quantitative basis for identifying novel targets for antiviral therapy. Our analysis suggests that HIV-1 assembly depending on Gag and Tat-Rev regulation of transcription and mRNA distribution present two most critical stages in HIV-1 replication that can be targeted to effectively control virus production. These processes are not covered by current antiretroviral treatments.
• dc.format.mimetype application/pdf
• dc.identifier.citation Shcherbatova O, Grebennikov D, Sazonov I, Meyerhans A, Bocharov G. Modeling of the HIV-1 life cycle in productively infected cells to predict novel therapeutic targets. Pathogens. 2020; 9(4). pii: E255. DOI: 10.3390/pathogens9040255
• dc.identifier.doi http://dx.doi.org/10.3390/pathogens9040255
• dc.identifier.issn 2076-0817
• dc.identifier.uri http://hdl.handle.net/10230/44423
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Pathogens. 2020; 9(4). pii: E255
• dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword HIV-1
• dc.subject.keyword Antiviral targets
• dc.subject.keyword Intracellular replication
• dc.subject.keyword Mathematical model
• dc.subject.keyword Sensitivity analysis
• dc.title Modeling of the HIV-1 life cycle in productively infected cells to predict novel therapeutic targets
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion