Transcriptomic analysis of planarians under simulated microgravity or 8 g demonstrates that alteration of gravity induces genomic and cellular alterations that could facilitate tumoral transformation

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author de Sousa, Nídia
• dc.contributor.author Rodríguez Esteban, Gustavo
• dc.contributor.author Colagè, Ivan
• dc.contributor.author D'Ambrosio, Paolo
• dc.contributor.author van Loon, Jack J.W.A.
• dc.contributor.author Saló, Emili
• dc.contributor.author Adell, Teresa
• dc.contributor.author Auletta, Gennaro
• dc.date.accessioned 2019-03-06T08:36:59Z
• dc.date.available 2019-03-06T08:36:59Z
• dc.date.issued 2019
• dc.description.abstract The possibility of humans to live outside of Earth on another planet has attracted the attention of numerous scientists around the world. One of the greatest difficulties is that humans cannot live in an extra-Earth environment without proper equipment. In addition, the consequences of chronic gravity alterations in human body are not known. Here, we used planarians as a model system to test how gravity fluctuations could affect complex organisms. Planarians are an ideal system, since they can regenerate any missing part and they are continuously renewing their tissues. We performed a transcriptomic analysis of animals submitted to simulated microgravity (Random Positioning Machine, RPM) (s-µg) and hypergravity (8 g), and we observed that the transcriptional levels of several genes are affected. Surprisingly, we found the major differences in the s-µg group. The results obtained in the transcriptomic analysis were validated, demonstrating that our transcriptomic data is reliable. We also found that, in a sensitive environment, as under Hippo signaling silencing, gravity fluctuations potentiate the increase in cell proliferation. Our data revealed that changes in gravity severely affect genetic transcription and that these alterations potentiate molecular disorders that could promote the development of multiple diseases such as cancer.
• dc.description.sponsorship The possibility of humans to live outside of Earth on another planet has attracted the attention of numerous scientists around the world. One of the greatest difficulties is that humans cannot live in an extra-Earth environment without proper equipment. In addition, the consequences of chronic gravity alterations in human body are not known. Here, we used planarians as a model system to test how gravity fluctuations could affect complex organisms. Planarians are an ideal system, since they can regenerate any missing part and they are continuously renewing their tissues. We performed a transcriptomic analysis of animals submitted to simulated microgravity (Random Positioning Machine, RPM) (s-µg) and hypergravity (8 g), and we observed that the transcriptional levels of several genes are affected. Surprisingly, we found the major differences in the s-µg group. The results obtained in the transcriptomic analysis were validated, demonstrating that our transcriptomic data is reliable. We also found that, in a sensitive environment, as under Hippo signaling silencing, gravity fluctuations potentiate the increase in cell proliferation. Our data revealed that changes in gravity severely affect genetic transcription and that these alterations potentiate molecular disorders that could promote the development of multiple diseases such as cancer.
• dc.format.mimetype application/pdf
• dc.identifier.citation de Sousa N, Rodriguez-Esteban G, Colagè I, D'Ambrosio P, van Loon JJWA, Saló E et al. Transcriptomic analysis of planarians under simulated microgravity or 8 g demonstrates that alteration of gravity induces genomic and cellular alterations that could facilitate tumoral transformation. Int J Mol Sci. 2019;20(3):720. DOI: 10.3390/ijms20030720
• dc.identifier.doi http://dx.doi.org/10.3390/ijms20030720
• dc.identifier.issn 1422-0067
• dc.identifier.uri http://hdl.handle.net/10230/36752
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof International Journal of Molecular Sciences. 2019;20(3):720
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-56055-P
• dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Development
• dc.subject.keyword Hypergravity
• dc.subject.keyword Human health
• dc.subject.keyword Microgravity
• dc.subject.keyword Planarian
• dc.subject.keyword Regeneration
• dc.subject.keyword Space exploration
• dc.subject.keyword Transcriptomics
• dc.subject.keyword Random Positioning Machine (RPM)
• dc.subject.keyword Large Diameter Centrifuge (LDC)
• dc.title Transcriptomic analysis of planarians under simulated microgravity or 8 g demonstrates that alteration of gravity induces genomic and cellular alterations that could facilitate tumoral transformation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion