Hog1 bypasses stress-mediated down-regulation of transcription by RNA polymerase II redistribution and chromatin remodeling
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- dc.contributor.author Nadal Ribelles, Mariona, 1984-ca
- dc.contributor.author Conde Pueyo, Núria, 1983-ca
- dc.contributor.author Flores, Oscarca
- dc.contributor.author González-Vallinas Rostes, Juan, 1983-ca
- dc.contributor.author Eyras Jiménez, Eduardoca
- dc.contributor.author Nadal Clanchet, Eulàlia deca
- dc.contributor.author Posas Garriga, Francescca
- dc.date.accessioned 2013-11-28T11:32:23Z
- dc.date.available 2013-11-28T11:32:23Z
- dc.date.issued 2012ca
- dc.description.abstract Cells are subjected to dramatic changes of gene expression upon environmental changes. Stress/ncauses a general down-regulation of gene expression together with the induction of a set of stress-responsive/ngenes. The p38-related stress-activated protein kinase Hog1 is an important regulator of transcription upon/nosmostress in yeast. Genome-wide localization studies of RNA polymerase II (RNA Pol II) and Hog1 showed that stress induced major changes in RNA Pol II localization, with a shift toward stress-responsive genes relative to housekeeping genes. RNA Pol II relocalization required Hog1, which was also localized to stress-responsive loci. In addition to RNA Pol II-bound genes, Hog1 also localized to RNA polymerase III-bound genes, pointing to a wider role for Hog1 in transcriptional control than initially expected. Interestingly, an increasing association of Hog1 with stressresponsive genes was strongly correlated with chromatin remodeling and increased gene expression. Remarkably, MNase-Seq analysis showed that although chromatin structure was not significantly altered at a genome-wide level in response to stress, there was pronounced chromatin remodeling for those genes that displayed Hog1 association. Hog1 serves to bypass the general down-regulation of gene expression that occurs in response to osmostress, and does so both by targeting RNA Pol II machinery and by inducing chromatin remodeling at stressresponsive loci.en
- dc.description.sponsorship MN is a recipient of an FIS fellowship. This work was supported by grants from the Spanish Government (BIO2011-23920 to EE, BIO2009-07762 and BFU2012-33503 to FP, BFU2011-26722 to EN), the Consolider Ingenio 2010/nprogramme CSD2007-0015 to FP and FP7 UNICELLSYS grant 201142, the Fundación Marcelino Botín (FMB) to FP. EN and FP are recipients of an ICREA Acadèmia (Generalitat de Catalunya).en
- dc.format.mimetype application/pdfca
- dc.identifier.citation Nadal-Ribelles M, Conde N, Flores O, González-Vallinas J, Eyras E, Orozco M, et al. Hog1 bypasses stress-mediated down-regulation of transcription by RNA polymerase II redistribution and chromatin remodeling. Genome Biol. 2012;13(11):R106. DOI: 10.1186/gb-2012-13-11-r106ca
- dc.identifier.doi http://dx.doi.org/10.1186/gb-2012-13-11-r106
- dc.identifier.issn 1465-6906ca
- dc.identifier.uri http://hdl.handle.net/10230/21315
- dc.language.iso engca
- dc.publisher BioMed Centralca
- dc.relation.ispartof Genome biology. 2012;13(11):R106
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2011-23920
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2009-07762
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-33503
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-26722
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/CSD2007-0015
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/201142
- dc.rights © Nadal-Ribelles et al. Creative Commons Attribution Licenseca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/2.0/
- dc.title Hog1 bypasses stress-mediated down-regulation of transcription by RNA polymerase II redistribution and chromatin remodelingca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca