Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals

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• dc.contributor.author Gentsch, George E.
• dc.contributor.author Spruce, Thomas
• dc.contributor.author Owens, Nick D.L.
• dc.contributor.author Smith, James
• dc.date.accessioned 2020-04-03T07:28:30Z
• dc.date.available 2020-04-03T07:28:30Z
• dc.date.issued 2019
• dc.description.abstract Embryonic development yields many different cell types in response to just a few families of inductive signals. The property of signal-receiving cells that determines how they respond to inductive signals is known as competence, and it differs in different cell types. Here, we explore the ways in which maternal factors modify chromatin to specify initial competence in the frog Xenopus tropicalis. We identify early-engaged regulatory DNA sequences, and infer from them critical activators of the zygotic genome. Of these, we show that the pioneering activity of the maternal pluripotency factors Pou5f3 and Sox3 determines competence for germ layer formation by extensively remodelling compacted chromatin before the onset of inductive signalling. This remodelling includes the opening and marking of thousands of regulatory elements, extensive chromatin looping, and the co-recruitment of signal-mediating transcription factors. Our work identifies significant developmental principles that inform our understanding of how pluripotent stem cells interpret inductive signals.
• dc.format.mimetype application/pdf
• dc.identifier.citation Gentsch GE, Spruce T, Owens NDL, Smith JC. Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals. Nat Commun. 2019; 10(1):4269. DOI: 10.1038/s41467-019-12263-w
• dc.identifier.doi http://dx.doi.org/10.1038/s41467-019-12263-w
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/44152
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Nat Commun. 2019; 10(1):4269
• dc.rights © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Chromatin
• dc.subject.keyword Embryonic induction
• dc.subject.keyword Gene expression
• dc.subject.keyword Pluripotency
• dc.subject.keyword Transcription
• dc.title Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion