EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer
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- dc.contributor.author Roman-Canal, Berta
- dc.contributor.author Tarragona, Jordi
- dc.contributor.author Moiola, Cristian Pablo
- dc.contributor.author Gatius, Sònia
- dc.contributor.author Bonnin, Sarah
- dc.contributor.author Ruiz-Miró, Maria
- dc.contributor.author Sierra, José Enrique
- dc.contributor.author Rufas, Maria
- dc.contributor.author González, Esperanza
- dc.contributor.author Porcel, José M.
- dc.contributor.author Gil-Moreno, Antonio
- dc.contributor.author Falcón-Pérez, Juan M.
- dc.contributor.author Ponomarenko, Julia
- dc.contributor.author Matias-Guiu, Xavier
- dc.contributor.author Colas, Eva
- dc.date.accessioned 2019-09-20T10:53:25Z
- dc.date.available 2019-09-20T10:53:25Z
- dc.date.issued 2019
- dc.description.abstract Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
- dc.format.mimetype application/pdf
- dc.identifier.citation Roman-Canal B, Tarragona J, Moiola CP, Gatius S, Bonnin S, Ruiz-Miró M et al. EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer. J Transl Med. 2019;17(1):208. DOI: 10.1186/s12967-019-1954-8
- dc.identifier.doi http://dx.doi.org/10.1186/s12967-019-1954-8
- dc.identifier.issn 1479-5876
- dc.identifier.uri http://hdl.handle.net/10230/42305
- dc.language.iso eng
- dc.publisher BioMed Central
- dc.relation.ispartof Journal of Translational Medicine. 2019;17(1):208
- dc.rights © 2019, Berta Roman-Canal et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Colorectal cancer
- dc.subject.keyword Biomarkers
- dc.subject.keyword Diagnostic
- dc.subject.keyword miRNAs
- dc.subject.keyword Ascitic fluid
- dc.subject.keyword Peritoneal lavage
- dc.subject.keyword Liquid biopsy
- dc.subject.keyword Extracellular vesicles
- dc.subject.keyword Colon cancer
- dc.title EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion