Storage conditions and stability of global DNA methylation in placental tissue
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- dc.contributor.author Vilahur Chiaraviglio, Nadia, 1982-
- dc.contributor.author Baccarelli, Andrea A.
- dc.contributor.author Bustamante Pineda, Mariona
- dc.contributor.author Agramunt, Silvia
- dc.contributor.author Byun, Hyang-Min
- dc.contributor.author Fernandez, Mariana F.
- dc.contributor.author Sunyer Deu, Jordi
- dc.contributor.author Estivill, Xavier, 1955-
- dc.date.accessioned 2019-02-15T06:28:15Z
- dc.date.available 2019-02-15T06:28:15Z
- dc.date.issued 2013
- dc.description.abstract AIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30 min, 1 h, 2 h, 6 h and 24 h. Global DNA methylation was measured by bisulfite pyrosequencing of repetitive elements and the luminometric methylation assay. RESULTS: Small changes in global DNA methylation in relation to time-to-storage were observed by pyrosequencing, with a coefficient of variation (COV) of 2.49% (placenta 1) and 2.86% (placenta 2), similar to the mean technical variation observed for pyrosequencing (COV: 1.91 and 1.51%, respectively). A luminometric methylation assay yielded more variable results in the two placentas analyzed, both among time points (COV: 9.13 and 10.35%, respectively) and technical replicates (COV: 11.60 and 9.80%, respectively). CONCLUSION: Global DNA methylation is stable at room temperature. However, some techniques to measure methylation might be confounded by DNA degradation caused by a delay in storage.
- dc.description.sponsorship This study was funded by grants from the Spanish Ministry of Health (FIS-PI041436 and PI11/00610), Instituto de Salud Carlos III (Red Infancia y MedioAmbiente G03/176 and CB06/02/0041) and the Generalitat de Catalunya-Comissió Interdepartamental de Recerca i Innovació Tecnològica 1999SGR 00241. AA Baccarelli receives support from the Harvard School of Public Health and National Institute of Environmental Health Sciences Center for Environmental Health (ES000002). N Vilahur was supported by a Formación de Personal Investigador Grant from the Spanish Ministry of Health and a Formación de Personal Investigador Grant for Short Research Stays in Foreign Institutions (BES-2009-023933) and MF Fernandez by the Ramon y Cajal Research Grant from the Spanish Ministry of Education
- dc.format.mimetype application/pdf
- dc.identifier.citation Vilahur N, Baccarelli AA, Bustamante M, Agramunt S, Byun HM, Fernandez MF et al. Storage conditions and stability of global DNA methylation in placental tissue. Epigenomics. 2013 Jun;5(3):341-8. DOI: 10.2217/epi.13.29
- dc.identifier.doi http://dx.doi.org/10.2217/epi.13.29
- dc.identifier.issn 1750-1911
- dc.identifier.uri http://hdl.handle.net/10230/36592
- dc.language.iso eng
- dc.publisher Future Medicine
- dc.relation.ispartof Epigenomics. 2013 Jun;5(3):341-8
- dc.rights © 2013 Future Medicine Ltd
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.other ADN -- Metabolisme
- dc.subject.other ADN -- Metilació
- dc.subject.other Placenta -- Metabolisme
- dc.title Storage conditions and stability of global DNA methylation in placental tissue
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion