Virulence genes and subclone status as markers of experimental virulence in a murine sepsis model among Escherichia coli sequence type 131 clinical isolates from Spain
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- dc.contributor.author Merino, Irene
- dc.contributor.author Porter, Stephen B.
- dc.contributor.author Johnston, Brian D.
- dc.contributor.author Clabots, Connie
- dc.contributor.author Shaw, Evelyn
- dc.contributor.author Horcajada Gallego, Juan Pablo
- dc.contributor.author Cantón, Rafael
- dc.contributor.author Ruiz-Garbajosa, Patricia
- dc.contributor.author Johnson, James R.
- dc.contributor.author ITUBRAS-GEIH group
- dc.date.accessioned 2019-01-15T08:04:38Z
- dc.date.available 2019-01-15T08:04:38Z
- dc.date.issued 2017
- dc.description.abstract OBJECTIVE: To assess experimental virulence among sequence type 131 (ST131) Escherichia coli bloodstream isolates in relation to virulence genotype and subclone. METHODS: We analysed 48 Spanish ST131 bloodstream isolates (2010) by PCR for ST131 subclone status (H30Rx, H30 non-Rx, or non-H30), virulence genes (VGs), and O-type. Then we compared these traits with virulence in a murine sepsis model, as measured by illness severity score (ISS) and rapid lethality (mean ISS ≥ 4). RESULTS: Of the 48 study isolates, 65% were H30Rx, 21% H30 non-Rx, and 15% non-H30; 44% produced ESBLs, 98% were O25b, and 83% qualified as extraintestinal pathogenic E. coli (ExPEC). Of 49 VGs, ibeA and iss were associated significantly with non-H30 isolates, and sat, iha and malX with H30 isolates. Median VG scores differed by subclone, i.e., 12 (H30Rx), 10 (H30 non-Rx), and 11 (non-H30) (p < 0.01). Nearly 80% of isolates represented a described virotype. In mice, H30Rx and non-H30 isolates were more virulent than H30 non-Rx isolates (according to ISS [p = 0.03] and rapid lethality [p = 0.03]), as were ExPEC isolates compared with non-ExPEC isolates (median ISS, 4.3 vs. 2.7: p = 0.03). In contrast, most individual VGs, VG scores, VG profiles, and virotypes were not associated with mouse virulence. CONCLUSIONS: ST131 subclone and ExPEC status, but not individual VGs, VG scores or profiles, or virotypes, predicted mouse virulence. Given the lower virulence of non-Rx H30 isolates, hypervirulence probably cannot explain the ST131-H30 clade's epidemic emergence.
- dc.format.mimetype application/pdf
- dc.identifier.citation Merino I, Porter SB, Johnston BD, Clabots C, Shaw E, Horcajada JP. Et al. Virulence genes and subclone status as markers of experimental virulence in a murine sepsis model among Escherichia coli sequence type 131 clinical isolates from Spain. PLoS One. 2017 Nov 30;12(11):e0188838. DOI: 10.1371/journal.pone.0188838
- dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0188838
- dc.identifier.issn 1932-6203
- dc.identifier.uri http://hdl.handle.net/10230/36264
- dc.language.iso eng
- dc.publisher Public Library of Science (PLoS)
- dc.relation.ispartof PLoS One. 2017 Nov 30;12(11):e0188838
- dc.rights Copyright © 2017 Merino et al. This is an open access article distributed under the terms of the Creative Commons Attribution License,https://creativecommons.org/licenses/by/4.0/ which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.other Septicèmia
- dc.subject.other Escheríchia coli -- Genètica
- dc.subject.other Genètica bacteriana
- dc.title Virulence genes and subclone status as markers of experimental virulence in a murine sepsis model among Escherichia coli sequence type 131 clinical isolates from Spain
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion