Glycodendropeptides stimulate dendritic cell maturation and T cell proliferation: a potential influenza A virus immunotherapy

Mascaraque, Ainhoa; Kowalczyk, Wioleta; Fernández, Tahia; Palomares, Francisca; Mayorga, Cristobalina; Andreu Martínez, David; Rojo, Javier

Glycodendropeptides stimulate dendritic cell maturation and T cell proliferation: a potential influenza A virus immunotherapy

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dc.contributor.author Mascaraque, Ainhoaca
dc.contributor.author Kowalczyk, Wioletaca
dc.contributor.author Fernández, Tahiaca
dc.contributor.author Palomares, Franciscaca
dc.contributor.author Mayorga, Cristobalinaca
dc.contributor.author Andreu Martínez, Davidca
dc.contributor.author Rojo, Javierca
dc.date.accessioned 2016-02-01T15:27:51Z
dc.date.available 2016-02-01T15:27:51Z
dc.date.issued 2015
dc.description.abstract Mannosylation facilitates uptake and internalization of immunogenic peptides by antigen-processing cells expressing mannose receptors at their surface, such as DC-SIGN, a lectin that plays a key role in the immune response against different pathogens. This internalization, processing and subsequent MHC presentation may result in a strong T cell stimulation. Here, we hypothesized that combining mannose glycodendrons with multivalent presentation of peptide epitopes in a likewise dendron format would yield hybrid constructs, named glycodendropeptides (GDPs), with the capacity to enhance peptide immunogenicity, hence providing a novel and versatile platform for applications in immunotherapy. Thus, GDPs of different valencies displaying the NP366–374 epitope, a conserved sequence from the influenza A virus nucleoprotein (NP), have been built by two click chemistry-based methodologies and assessed as potential flu vaccine candidates. Preliminary evaluation of the ability of these constructs to stimulate dendritic cell maturation and lymphocyte proliferation was promising, showing the highest-functionalized NP366–374 GDPs as inducing the strongest immunostimulatory effect.ca
dc.description.sponsorship This work was supported by the Instituto de Salud Carlos III (ISCIII) Thematic Networks and Co-operative Research Centres: RIRAAF (RD012/0013/0001 and RD012/0013/0016) and project ISCIII (PI12/02481); by Junta de Andalucía (CTS-7433) and the Nicolas Monardes Program (C-0044-2012 SAS 2013); and by Generalitat de Catalunya (SGR2009-00492) and Ministerio de Economía y Competitividad (MINECO), projects CTQ2011-23410 and SAF2011-24899. This work was co-financed by the European Regional Development Fund (ERDF)
dc.format.mimetype application/pdfca
dc.identifier.citation Mascaraque A, Kowalczyk W, Fernández T, Palomares F, Mayorga C, Andreu D et al. Glycodendropeptides stimulate dendritic cell maturation and T cell proliferation: a potential influenza A virus immunotherapy. MedChemComm. 2015;6:1755-60. DOI: 10.1039/C5MD00133Aca
dc.identifier.doi http://dx.doi.org/10.1039/C5MD00133A
dc.identifier.issn 2040-2503
dc.identifier.uri http://hdl.handle.net/10230/25708
dc.language.iso engca
dc.publisher Royal Society of Chemistryca
dc.relation.ispartof MedChemComm. 2015;6:1755-60
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2011-24899
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri http://creativecommons.org/licenses/by-nc/3.0/ca
dc.subject.other Cèl·lules Tca
dc.subject.other Gripca
dc.title Glycodendropeptides stimulate dendritic cell maturation and T cell proliferation: a potential influenza A virus immunotherapyca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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