Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK

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• dc.contributor.author Tomas-Hernandez, Sarah
• dc.contributor.author Blanco, Jordi
• dc.contributor.author Garcia Vallvé, Santiago
• dc.contributor.author Pujadas, Gerard
• dc.contributor.author Ojeda-Montes, María José
• dc.contributor.author Gimeno, Aleix
• dc.contributor.author Arola, Lluís
• dc.contributor.author Minghetti, Luisa
• dc.contributor.author Beltrán-Debón, Raúl
• dc.contributor.author Mulero, Miquel
• dc.date.accessioned 2022-06-10T10:11:40Z
• dc.date.available 2022-06-10T10:11:40Z
• dc.date.issued 2021
• dc.description.abstract In response to foreign or endogenous stimuli, both microglia and astrocytes adopt an activated phenotype that promotes the release of pro-inflammatory mediators. This inflammatory mechanism, known as neuroinflammation, is essential in the defense against foreign invasion and in normal tissue repair; nevertheless, when constantly activated, this process can become detrimental through the release of neurotoxic factors that amplify underlying disease. In consequence, this study presents the anti-inflammatory and immunomodulatory properties of o-orsellinaldehyde, a natural compound found by an in silico approach in the Grifola frondosa mushroom, in astrocytes and microglia cells. For this purpose, primary microglia and astrocytes were isolated from mice brain and cultured in vitro. Subsequently, cells were exposed to LPS in the absence or presence of increasing concentrations of this natural compound. Specifically, the results shown that o-orsellinaldehyde strongly inhibits the LPS-induced inflammatory response in astrocytes and microglia by decreasing nitrite formation and downregulating iNOS and HO-1 expression. Furthermore, in microglia cells o-orsellinaldehyde inhibits NF-κB activation; and potently counteracts LPS-mediated p38 kinase and JNK phosphorylation (MAPK). In this regard, o-orsellinaldehyde treatment also induces a significant cell immunomodulation by repolarizing microglia toward the M2 anti-inflammatory phenotype. Altogether, these results could partially explain the reported beneficial effects of G. frondosa extracts on inflammatory conditions.
• dc.description.sponsorship Funding: A.G.’s contract was supported by grant 2015FI_B00655 from the Catalonia Government
• dc.format.mimetype application/pdf
• dc.identifier.citation Tomas-Hernandez S, Blanco J, Garcia-Vallvé S, Pujadas G, Ojeda-Montes MJ, Gimeno A et al. Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK. Pharmaceutics. 2021 May 28;13(6):806. DOI:10.3390/pharmaceutics13060806
• dc.identifier.doi http://dx.doi.org/10.3390/pharmaceutics13060806
• dc.identifier.issn 1999-4923
• dc.identifier.uri http://hdl.handle.net/10230/53449
• dc.language.iso eng
• dc.publisher MDPI
• dc.rights © 2021 by Sarah Tomas-Hernandez et al. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.other Gírgola de castanyer
• dc.subject.other Antiinflamatoris
• dc.subject.other Genètica
• dc.title Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion