Composition and evolution of the vertebrate and mammalian selenoproteomes

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• dc.contributor.author Mariotti, Marco, 1984-ca
• dc.contributor.author Ridge, Perry G.ca
• dc.contributor.author Zhang, Yanca
• dc.contributor.author Lobanov, Alexei V.ca
• dc.contributor.author Pringle, Thomas H.ca
• dc.contributor.author Guigó Serra, Rodericca
• dc.contributor.author Hatfield, Dolph L.ca
• dc.contributor.author Gladyshev, Vadim N.ca
• dc.date.accessioned 2014-06-20T08:18:09Z
• dc.date.available 2014-06-20T08:18:09Z
• dc.date.issued 2012ca
• dc.description.abstract BACKGROUND: Selenium is an essential trace element in mammals due to its presence in proteins in the form of selenocysteine (Sec). Human genome codes for 25 Sec-containing protein genes, and mouse and rat genomes for 24. METHODOLOGY/PRINCIPAL FINDINGS: We characterized the selenoproteomes of 44 sequenced vertebrates by applying gene prediction and phylogenetic reconstruction methods, supplemented with the analyses of gene structures, alternative splicing isoforms, untranslated regions, SECIS elements, and pseudogenes. In total, we detected 45 selenoprotein subfamilies. 28 of them were found in mammals, and 41 in bony fishes. We define the ancestral vertebrate (28 proteins) and mammalian (25 proteins) selenoproteomes, and describe how they evolved along lineages through gene duplication (20 events), gene loss (10 events) and replacement of Sec with cysteine (12 events). We show that an intronless selenophosphate synthetase 2 gene evolved in early mammals and replaced functionally the original multiexon gene in placental mammals, whereas both genes remain in marsupials. Mammalian thioredoxin reductase 1 and thioredoxin-glutathione reductase evolved from an ancestral glutaredoxin-domain containing enzyme, still present in fish. Selenoprotein V and GPx6 evolved specifically in placental mammals from duplications of SelW and GPx3, respectively, and GPx6 lost Sec several times independently. Bony fishes were characterized by duplications of several selenoprotein families (GPx1, GPx3, GPx4, Dio3, MsrB1, SelJ, SelO, SelT, SelU1, and SelW2). Finally, we report identification of new isoforms for several selenoproteins and describe unusually conserved selenoprotein pseudogenes. CONCLUSIONS/SIGNIFICANCE: This analysis represents the first comprehensive survey of the vertebrate and mammal selenoproteomes, and depicts their evolution along lineages. It also provides a wealth of information on these selenoproteins and their forms.
• dc.description.sponsorship Supported by NIH GM061603 and GM065204
• dc.format.mimetype application/pdfca
• dc.identifier.citation Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R et al. Composition and evolution of the vertebrate and mammalian selenoproteomes. PLoS One. 2012;7(3):e33066. DOI: 10.1371/journal.pone.0033066ca
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0033066
• dc.identifier.issn 1932-6203ca
• dc.identifier.uri http://hdl.handle.net/10230/22590
• dc.language.iso engca
• dc.publisher Public Library of Science (PLoS)ca
• dc.relation.ispartof PLoS One. 2012;7(3):e33066
• dc.rights © 2012 Marco Mariotti et al. This is an Open Access article distributed under the terms of the Creative Commons Attributionca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.5/
• dc.subject.other Vertebrats -- Metabolisme
• dc.subject.other Mamífers -- Metabolisme
• dc.subject.other Proteïnes
• dc.title Composition and evolution of the vertebrate and mammalian selenoproteomesca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca