Polymer modeling of the E. coli genome reveals the involvement of locus positioning and macrodomain structuring for the control of chromosome conformation and segregation

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• dc.contributor.author Junier, Ivan
• dc.contributor.author Boccard, Frédéric
• dc.contributor.author Espéli, Olivier
• dc.date.accessioned 2024-01-29T07:20:15Z
• dc.date.available 2024-01-29T07:20:15Z
• dc.date.issued 2014
• dc.description.abstract The mechanisms that control chromosome conformation and segregation in bacteria have not yet been elucidated. In Escherichia coli, the mere presence of an active process remains an open question. Here, we investigate the conformation and segregation pattern of the E. coli genome by performing numerical simulations on a polymer model of the chromosome. We analyze the roles of the intrinsic structuring of chromosomes and the forced localization of specific loci, which are observed in vivo. Specifically, we examine the segregation pattern of a chromosome that is divided into four structured macrodomains (MDs) and two non-structured regions. We find that strong osmotic-like organizational forces, which stem from the differential condensation levels of the chromosome regions, dictate the cellular disposition of the chromosome. Strikingly, the comparison of our in silico results with fluorescent imaging of the chromosome choreography in vivo reveals that in the presence of MDs the targeting of the origin and terminus regions to specific positions are sufficient to generate a segregation pattern that is indistinguishable from experimentally observed patterns.
• dc.format.mimetype application/pdf
• dc.identifier.citation Junier I, Boccard F, Espéli O. Polymer modeling of the E. coli genome reveals the involvement of locus positioning and macrodomain structuring for the control of chromosome conformation and segregation. Nucleic Acids Research. 2014 Feb 1;42(3):1461-73. DOI: 10.1093/nar/gkt1005
• dc.identifier.doi http://dx.doi.org/10.1093/nar/gkt1005
• dc.identifier.issn 0305-1048
• dc.identifier.uri http://hdl.handle.net/10230/58844
• dc.language.iso eng
• dc.publisher Oxford University Press
• dc.relation.ispartof Nucleic Acids Research. 2014 Feb 1;42(3):1461-73
• dc.rights © The Author(s) 2013. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/3.0/
• dc.subject.other Cromosomes
• dc.subject.other Escheríchia coli
• dc.subject.other Genomes bacterians
• dc.title Polymer modeling of the E. coli genome reveals the involvement of locus positioning and macrodomain structuring for the control of chromosome conformation and segregation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion