Methionine sulphoxide reductases revisited: free methionine as a primary target of H2O2 stress in auxotrophic fission yeast

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• dc.contributor.author García Santamarina, Sarela, 1978-ca
• dc.contributor.author Boronat i Llop, Susanna, 1965-ca
• dc.contributor.author Ayté del Olmo, Joséca
• dc.contributor.author Hidalgo Hernando, Elenaca
• dc.date.accessioned 2016-01-14T14:58:18Z
• dc.date.available 2016-01-14T14:58:18Z
• dc.date.issued 2013
• dc.description.abstract Amino acid methionine can suffer reversible oxidation to sulphoxide and further irreversible over-oxidation to methionine sulphone. As part of the cellular antioxidant scavenging activities are the methionine sulphoxide reductases (Msrs), with a reported role in methionine sulphoxide reduction, both free and in proteins. Three families of Msrs have been described, but the fission yeast genome only includes one representative for two of these families: MsrA/Mxr1 and MsrB/Mxr2. We have investigated their role in methionine reduction and H2 O2 sensitivity. We show here that MsrA/Mxr1 is able to reduce free oxidized methionine. Cells lacking each one of the genes are not significantly sensitive to different types of oxidative stresses, neither display altered life span. However, only when deletion of msrA/mxr1 is combined with deletion of met6, which confers methionine auxotrophy, the survival upon H2 O2 stress decreases by 100-fold. In fact, cells lacking only Met6, and which therefore require addition of methionine to the growth media, are extremely sensitive to H2 O2 stress. These and other evidences suggest that oxidation of free methionine is a primary target of peroxide toxicity in cells devoid of methionine biosynthetic capacity, and that an important role of Msrs is to recycle this oxidized free amino acid.ca
• dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Innovation (BFU2009-06933, BFU2012-32045), PLAN E and FEDER, by the Spanish program Consolider-Ingenio 2010 Grant CSD 2007-0020 and by SGR 2009-195 from Generalitat de Catalunya (Spain) to E.H. E.H. and J.A. are recipients of ICREA Academia Awards (Generalitat de Catalunya). The authors declare no conflict of interests.
• dc.format.mimetype application/pdfca
• dc.identifier.citation García-Santamarina S, Boronat S, Ayté J, Hidalgo E. Methionine sulphoxide reductases revisited: free methionine as a primary target of H2O2 stress in auxotrophic fission yeast. Mol Microbiol. 2013;90(5):1113-24. DOI: 10.1111/mmi.12420ca
• dc.identifier.doi http://dx.doi.org/10.1111/mmi.12420
• dc.identifier.issn 0950-382X
• dc.identifier.uri http://hdl.handle.net/10230/25576
• dc.language.iso engca
• dc.publisher Wiley-Blackwellca
• dc.relation.ispartof Molecular microbiology. 2013;90(5):1113-24
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-06933
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-32045
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/CSD2007-0020
• dc.rights © 2013 John Wiley & Sons Ltd. This is the pre-peer reviewed version of the following article: García-Santamarina S, Boronat S, Ayté J, Hidalgo E. Methionine sulphoxide reductases revisited: free methionine as a primary target of H2O2 stress in auxotrophic fission yeast. Molecular microbiology. 2013, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/mmi.12420/abstractca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.keyword Methionine
• dc.subject.keyword Methionine oxidation
• dc.subject.keyword H2O2 reactivity
• dc.subject.keyword Methionine sulfoxide reductases
• dc.subject.keyword 22 antioxidant defenses
• dc.subject.other Schizosaccharomyces pombe -- Metabolismeca
• dc.subject.other Reacció d'oxidació-reduccióca
• dc.title Methionine sulphoxide reductases revisited: free methionine as a primary target of H2O2 stress in auxotrophic fission yeastca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca