Allosteric inhibition of Aurora-A kinase by a synthetic vNAR domain
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- dc.contributor.author Burgess, Selena G.ca
- dc.contributor.author Oleksy, Arkadiuszca
- dc.contributor.author Cavazza, Tommaso, 1985-ca
- dc.contributor.author Richards, Mark W.ca
- dc.contributor.author Vernos, Isabelle, 1959-ca
- dc.contributor.author Matthews, Davidca
- dc.contributor.author Bayliss, Richardca
- dc.date.accessioned 2017-01-26T13:34:58Z
- dc.date.available 2017-01-26T13:34:58Z
- dc.date.issued 2016ca
- dc.description.abstract The vast majority of clinically approved protein kinase inhibitors target the ATP-binding pocket directly. Consequently, many inhibitors have broad selectivity profiles and most have significant off-target effects. Allosteric inhibitors are generally more selective, but are difficult to identify because allosteric binding sites are often unknown or poorly characterized. Aurora-A is activated through binding of TPX2 to an allosteric site on the kinase catalytic domain, and this knowledge could be exploited to generate an inhibitor. Here, we generated an allosteric inhibitor of Aurora-A kinase based on a synthetic, vNAR single domain scaffold, vNAR-D01. Biochemical studies and a crystal structure of the Aurora-A/vNAR-D01 complex show that the vNAR domain overlaps with the TPX2 binding site. In contrast with the binding of TPX2, which stabilizes an active conformation of the kinase, binding of the vNAR domain stabilizes an inactive conformation, in which the αC-helix is distorted, the canonical Lys-Glu salt bridge is broken and the regulatory (R-) spine is disrupted by an additional hydrophobic side chain from the activation loop. These studies illustrate how single domain antibodies can be used to characterize the regulatory mechanisms of kinases and provide a rational basis for structure-guided design of allosteric Aurora-A kinase inhibitors.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Burgess SG, Oleksy A, Cavazza T, Richards MW, Vernos I, Matthews D, Bayliss R. Allosteric inhibition of Aurora-A kinase by a synthetic vNAR domain. Open Biology. 2016;6(7):160089. DOI: 10.1098/rsob.160089ca
- dc.identifier.doi http://dx.doi.org/10.1098/rsob.160089
- dc.identifier.issn 2046-2441ca
- dc.identifier.uri http://hdl.handle.net/10230/27996
- dc.language.iso engca
- dc.publisher Royal Societyca
- dc.relation.ispartof Open Biology. 2016;6(7):160089
- dc.rights © 2016 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Antibody-assisted drug discovery
- dc.subject.keyword Structural biology
- dc.subject.keyword Biochemistry
- dc.subject.keyword Protein kinase
- dc.title Allosteric inhibition of Aurora-A kinase by a synthetic vNAR domainca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca