Juvenile hormone biosynthesis in adult Blattella germanica requires nuclear receptors Seven-up and FTZ-F1

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  • dc.contributor.author Borras-Castells, Ferran
  • dc.contributor.author Nieva, Claudia
  • dc.contributor.author Maestro, José L.
  • dc.contributor.author Maestro, Oscar
  • dc.contributor.author Bellés, Xavier, 1952-
  • dc.contributor.author Martín, David
  • dc.date.accessioned 2023-11-20T08:23:11Z
  • dc.date.available 2023-11-20T08:23:11Z
  • dc.date.issued 2017
  • dc.description Supplementary material for the online appendix.
  • dc.description.abstract In insects, the transition from juvenile development to the adult stage is controlled by juvenile hormone (JH) synthesized from the corpora allata (CA) glands. Whereas a JH-free period during the last juvenile instar triggers metamorphosis and the end of the growth period, the reappearance of this hormone after the imaginal molt marks the onset of reproductive adulthood. Despite the importance of such transition, the regulatory mechanism that controls it remains mostly unknown. Here, using the hemimetabolous insect Blattella germanica, we show that nuclear hormone receptors Seven-up-B (BgSvp-B) and Fushi tarazu-factor 1 (BgFTZ-F1) have essential roles in the tissue- and stage-specific activation of adult CA JH-biosynthetic activity. Both factors are highly expressed in adult CA cells. Moreover, RNAi-knockdown of either BgSvp-B or BgFTZ-F1 results in adult animals with a complete block in two critical JH-dependent reproductive processes, vitellogenesis and oogenesis. We show that this reproductive blockage is the result of a dramatic impairment of JH biosynthesis, due to the CA-specific reduction in the expression of two key JH biosynthetic enzymes, 3-hydroxy-3-methylglutaryl coenzyme A synthase-1 (BgHMG-S1) and HMG-reductase (BgHMG-R). Our findings provide insights into the regulatory mechanisms underlying the specific changes in the CA gland necessary for the proper transition to adulthood.
  • dc.description.sponsorship Support for this research was provided by the Spanish MINECO (grants BFU2009-10571 and CGL2014-55786-P to D.M. and CGL2012-36251 and CGL2015-64727-P to X.B.) and by the Catalan Government (2014 SGR 619 to D.M., J.L.M. and X.B.). The research has also benefited from FEDER funds. F.B.-C. is a recipient of a pre-doctoral research Grant from the MICINN. C.N. is a recipient of a postdoctoral research grant from CSIC. O.M. is recipient of a pre-doctoral research grant from Generalitat de Catalunya.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Borras-Castells F, Nieva C, Maestro JL, Maestro O, Belles X, Martín D. Juvenile hormone biosynthesis in adult Blattella germanica requires nuclear receptors Seven-up and FTZ-F1. Sci Rep. 2017 Jan 11;7:40234. DOI: 10.1038/srep40234
  • dc.identifier.doi http://dx.doi.org/10.1038/srep40234
  • dc.identifier.issn 2045-2322
  • dc.identifier.uri http://hdl.handle.net/10230/58305
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.relation.ispartof Scientific Reports. 2017 Jan 11;7:40234
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-10571
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CGL2014-55786-P
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CGL2012-36251
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CGL2015-64727-P
  • dc.rights © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Differentiation
  • dc.subject.keyword RNAi
  • dc.title Juvenile hormone biosynthesis in adult Blattella germanica requires nuclear receptors Seven-up and FTZ-F1
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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