Visualizing the genome in high resolution challenges our textbook understanding

Lakadamyali, Melike; Cosma, Maria Pia, 1970-

doi:http://dx.doi.org/10.1038/s41592-020-0758-3

Visualizing the genome in high resolution challenges our textbook understanding

Citation

Lakadamyali M, Cosma M P. Visualizing the genome in high resolution challenges our textbook understanding. Nat Methods. 2020;17(4):371-9. DOI: 10.1038/s41592-020-0758-3

Abstract

The relationship between the 4D folding of the genome and its function is an outstanding question in biology. A range of methods that probe the folding of the genome in space and time with unprecedented resolution have been developed. These methods, including chromosome conformation capture and high-resolution light and electron microscopy, are shedding new light on genome architecture and function. Here, we review the emerging picture of genome organization revealed by super-resolution and live-cell imaging. We compare and contrast population-based chromosome conformation capture approaches and imaging-based approaches and highlight future challenges.