Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Lluch-Senar, Maria 1982-ca
- dc.contributor.author Delgado Blanco, Javierca
- dc.contributor.author Chen, Wei-Huaca
- dc.contributor.author Lloréns Rico, Verónica, 1989-ca
- dc.contributor.author O’Reilly, Francis J.ca
- dc.contributor.author Wodke, Judith A.H.ca
- dc.contributor.author Unal, E. Besrayca
- dc.contributor.author Yus, Evaca
- dc.contributor.author Martínez, Siraca
- dc.contributor.author Nichols, Robert J.ca
- dc.contributor.author Ferrar, Tonyca
- dc.contributor.author Vivancos Prellezo, Anaca
- dc.contributor.author Schmeisky, Arneca
- dc.contributor.author Stülke, Jörgca
- dc.contributor.author Noort, Vera vanca
- dc.contributor.author Gavin, Anne-Claudeca
- dc.contributor.author Bork, Peerca
- dc.contributor.author Serrano Pubull, Luis, 1982-ca
- dc.date.accessioned 2015-11-16T14:33:37Z
- dc.date.available 2015-11-16T14:33:37Z
- dc.date.issued 2015
- dc.description.abstract Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found that small ORFs (smORFs; < 100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%), followed by conventional ORFs (49%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual domains and not entire proteins, a notion we could confirm by expression of truncated domains. The fraction of essential non-coding RNAs (ncRNAs) non-overlapping with essential genes is 5% higher than of non-transcribed regions (0.9%), pointing to the important functions of the former. We found that the minimal essential genome is comprised of 33% (269,410 bp) of the M. pneumoniae genome. Our data highlight an unexpected hidden layer of smORFs with essential functions, as well as non-coding regions, thus changing the focus when aiming to define the minimal essential genome.en
- dc.format.mimetype application/pdfca
- dc.identifier.citation Lluch-Senar M, Delgado J, Chen WH, Lloréns-Rico V, O'Reilly FJ, Wodke JA et al. Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium. Molecular systems biology. 2015;11(1):780. DOI: 10.15252/msb.20145558ca
- dc.identifier.doi http://dx.doi.org/10.15252/msb.20145558
- dc.identifier.issn 1744-4292
- dc.identifier.uri http://hdl.handle.net/10230/25104
- dc.language.iso engca
- dc.publisher Wiley Blackwellca
- dc.relation.ispartof Molecular systems biology. 2015;11(1):780
- dc.rights © 2015 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/ca
- dc.subject.keyword Minimal genome
- dc.subject.keyword Non-coding RNAs
- dc.subject.keyword Small proteins
- dc.subject.other RNA no missatgersca
- dc.subject.other Interaccions RNA-proteïnaca
- dc.title Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacteriumen
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca