SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines

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  • dc.contributor.author Farfán, Nancy
  • dc.contributor.author Orellana Serradell, Octavio
  • dc.contributor.author Herrera, Daniela
  • dc.contributor.author Chrzanowsky, Dominique
  • dc.contributor.author Cubillos, Paulina
  • dc.contributor.author Marín, Gabriel
  • dc.contributor.author García de Herreros, Antonio
  • dc.contributor.author Castellón, Enrique A.
  • dc.contributor.author Contreras, Héctor R.
  • dc.date.accessioned 2020-04-20T07:34:25Z
  • dc.date.available 2020-04-20T07:34:25Z
  • dc.date.issued 2020
  • dc.description.abstract Zinc finger protein SNAI1 (SNAIL) and zinc finger protein SNAI2 (SLUG) transcription factors promote epithelial‑mesenchymal transition, a process through which epithelial cells acquire a mesenchymal phenotype, increasing their migratory and invasive properties. In prostate cancer (PCa) progression, increased expression levels of SNAIL and SLUG have been described. In advanced PCa, a decrease in the cell surface proteoglycan syndecan‑1 (SDC‑1), which has a role in cell‑to‑extracellular matrix adhesion, has been observed. Notably, SDC‑1 nuclear location has been observed in mesenchymal cancers. The present study aimed to determine if SNAIL and SLUG may be associated with the nuclear location of SDC‑1 in PCa. To determine the location of SDC‑1, antibodies against its intracellular domain (ID) or extracellular domain (ED) were used in benign prostatic hyperplasia (BPH) and PCa samples with high Gleason scores. Only ID‑SDC‑1 was located in the cell nuclei in advanced PCa samples, but not in the BPH samples. ED‑SDC‑1 was located in the cell membrane and cytoplasm, exhibiting decreased levels in PCa in comparison with those in BPH. Furthermore, LNCaP and PC3 PCa cell lines with ectopic SNAIL expression exhibited nuclear ID‑SDC‑1. No change was observed in the ED‑SDC‑1 levels, and maintained its location in the cell membrane and cytoplasm. SLUG induced no change in ID‑SDC‑1 location. At the protein level, an association between SNAIL and nuclear ID‑SDC‑1 was observed. In conclusion, the results of the present study demonstrated that nuclear ID‑SDC‑1 localization was associated with SNAIL expression in PCa cell lines.
  • dc.description.sponsorship The present study was supported by grants from FONDECYT awarded to HRC (grant nos. 1110269 and 1151214) and to EAC (grant no. 1140417), Grants from U-APOYA ENLACE, University of Chile (grant nos. ENL-22/19 and ENL 23/19), State Research Agency and the European Regional Development Fund (grant no. SAF2016-76461-R) awarded to AGH and the CONICYT (National Commission of Science and Technology) scholarship (grant no. 21140772) awarded to NF.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Farfán N, Orellana-Serradell O, Herrera D, Chrzanowsky D, Cubillos P, Marín G, Antonio García De Herreros A, Castellón EA, Contreras HR. SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines. Int J Mol Med. 2020; 45(4):1073-80. DOI: 10.3892/ijmm.2020.4488
  • dc.identifier.doi http://dx.doi.org/10.3892/ijmm.2020.4488
  • dc.identifier.issn 1107-3756
  • dc.identifier.uri http://hdl.handle.net/10230/44273
  • dc.language.iso eng
  • dc.publisher Spandidos Publications, Ltd.
  • dc.relation.ispartof Int J Mol Med. 2020; 45(4):1073-80
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-76461-R
  • dc.rights © Farfán et al. This is an open access article distributed under the terms of Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
  • dc.title SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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