Direct interaction between Id1 and Zrf1 controls neural differentiation of embryonic stem cells

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• dc.contributor.author Aloia, Luigica
• dc.contributor.author Gutiérrez, Arantxaca
• dc.contributor.author Martín Caballero, Juanca
• dc.contributor.author Di Croce, Lucianoca
• dc.date.accessioned 2016-07-22T12:57:44Z
• dc.date.available 2016-07-22T12:57:44Z
• dc.date.issued 2015
• dc.description.abstract Id proteins are dominant-negative regulators within the HLH family of proteins. In embryonic stem cells (ESCs), Id1 and Id3 maintain the pluripotent state by preventing neural differentiation. The Id1-interacting protein Zrf1 plays a crucial role as a chromatin-bound factor in specification of the neural fate from ESCs. Here, we show that Id1 blocks Zrf1 recruitment to chromatin, thus preventing the activation of neural genes in ESCs. Upon differentiation, Id1 expression decreases thus inducing Zrf1 binding to neural genes. Importantly, depletion of Zrf1 rescues the expression of Polycomb targets involved in neural specification which are up-regulated in Id1 knock-out ESCs. We therefore identified Zrf1 as transcriptional regulator of neural fate downstream of Id1 in ESCs.ca
• dc.description.sponsorship This work was supported by grants from the Spanish “Ministerio de Educación y Ciencia” (SAF2013-48926-P), from AGAUR, from AICR (10-0177), and from the European Commission’s 7th Framework Program 4DCellFate grant number 277899 to L.D.C
• dc.format.mimetype application/pdfca
• dc.identifier.citation Aloia L, Gutierrez A, Caballero JM, Di Croce L. Direct interaction between Id1 and Zrf1 controls neural differentiation of embryonic stem cells. EMBO Reports. 2015; 16(1): 63-70. DOI 10.15252/embr.201439560ca
• dc.identifier.doi http://dx.doi.org/10.15252/embr.201439560
• dc.identifier.issn 1469-221X
• dc.identifier.uri http://hdl.handle.net/10230/27107
• dc.language.iso engca
• dc.publisher Wiley-Blackwellca
• dc.relation.ispartof EMBO Reports. 2015; 16(1): 63-70
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/277899
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2013-48926-P
• dc.rights This is the pre-peer reviewed version of the following article: Aloia L, Gutierrez A, Caballero JM, Di Croce L. Direct interaction between Id1 and Zrf1 controls neural differentiation of embryonic stem cells. EMBO Reports. 2015; 16(1): 63-70. DOI 10.15252/embr.201439560, which has been published in final form at http://dx.doi.org/10.15252/embr.201439560. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.other Proteïnesca
• dc.subject.other Oncogensca
• dc.subject.other Cromatinaca
• dc.title Direct interaction between Id1 and Zrf1 controls neural differentiation of embryonic stem cellsca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca