The protective role of m1A during stress-induced granulation

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  • dc.contributor.author Alriquet, Marion
  • dc.contributor.author Calloni, Giulia
  • dc.contributor.author Martínez Limón, Adrián
  • dc.contributor.author Delli Ponti, Riccardo, 1987-
  • dc.contributor.author Hanspach, Gerd
  • dc.contributor.author Hegensbach, Martin
  • dc.contributor.author Tartaglia, Gian Gaetano
  • dc.contributor.author Vabulas, R. Martin
  • dc.date.accessioned 2020-10-15T09:38:06Z
  • dc.date.available 2020-10-15T09:38:06Z
  • dc.date.issued 2020
  • dc.description.abstract Post-transcriptional methylation of N6- and N1-adenine can effect transcriptome turnover and translation. Furthermore, the regulatory function of N6-methyladenine (m6A) during heat shock has been uncovered, including the enhancement of the phase separation potential of RNAs. In response to acute stress, e.g. heat shock, the orderly sequestration of mRNAs in stress granules (SGs) is considered important to protect transcripts from the irreversible aggregation. Until recently, the role of N1-methyladenine (m1A) on mRNAs during acute stress response remains largely unknown. Here we show that the methyltransferase complex TRMT6/61A, which generates the m1A tag, is involved in transcriptome protection during heat shock. Our bioinformatics analysis indicates that occurrence of the m1A motif is increased in mRNAs known to be enriched in SGs. Accordingly, the m1A-generating methyltransferase TRMT6/61A accumulated in SGs and mass spectrometry confirmed enrichment of m1A in the SG RNAs. The insertion of a single methylation motif in the untranslated region of a reporter RNA lead to more efficient recovery of protein synthesis from that transcript after the return to normal temperature. Our results demonstrate far-reaching functional consequences of a minimal RNA modification on N1-adenine during acute proteostasis stress.
  • dc.description.sponsorship This work was funded by the European Research Council grants MetaMeta_311522 (R.M.V.), RIBOMYLOME_309545 (G.G.T.) and ASTRA_855923 (G.G.T). G.G.T. acknowledges support of the H2020 projects IASIS_727658 and INFORE_825080, the Spanish Ministry of Economy and Competitiveness BFU2017-86970-P as well as the collaboration with Peter St. George-Hyslop financed by the Wellcome Trust. R.M.V. acknowledges support by the Deutsche Forschungsgemeinschaft (DFG) grant EXC115. M.H. is funded by DFG CRC902 “Molecular Principles of RNA-based Regulation”
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Alriquet M, Calloni G, Martínez-Limón A, Delli Ponti R, Hanspach G, Hengesbach M et al. The protective role of m1A during stress-induced granulation. J Mol Cell Biol. 2020;12(11):870-80. DOI: 10.1093/jmcb/mjaa023
  • dc.identifier.doi http://dx.doi.org/10.1093/jmcb/mjaa023
  • dc.identifier.issn 1674-2788
  • dc.identifier.uri http://hdl.handle.net/10230/45491
  • dc.language.iso eng
  • dc.publisher Oxford University Press (OUP)
  • dc.relation.ispartof Journal of Molecular Cell Biology. 2020;12(11):870-80
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/311522
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/309545
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/855923
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/727658
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/825080
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-86970-P
  • dc.rights © Marion Alriquet et al 2020. Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
  • dc.subject.other RNA
  • dc.subject.other Estrès -- Aspectes fisiològics
  • dc.subject.other Resposta per xoc tèrmic
  • dc.title The protective role of m1A during stress-induced granulation
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/acceptedVersion

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