Intravital imaging of hair-cell development and regeneration in the zebrafish

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• dc.contributor.author Pinto-Teixeira, Filipo
• dc.contributor.author Muzzopappa, Mariana
• dc.contributor.author Swoger, Jim
• dc.contributor.author Mineo, Alessandro
• dc.contributor.author Sharpe, James
• dc.contributor.author López-Schier, Hernán
• dc.date.accessioned 2024-10-22T06:38:18Z
• dc.date.available 2024-10-22T06:38:18Z
• dc.date.issued 2013
• dc.description.abstract Direct videomicroscopic visualization of organ formation and regeneration in toto is a powerful strategy to study cellular processes that often cannot be replicated in vitro. Intravital imaging aims at quantifying changes in tissue architecture or subcellular organization over time during organ development, regeneration or degeneration. A general feature of this approach is its reliance on the optical isolation of defined cell types in the whole animals by transgenic expression of fluorescent markers. Here we describe a simple and robust method to analyze sensory hair-cell development and regeneration in the zebrafish lateral line by high-resolution intravital imaging using laser-scanning confocal microscopy (LSCM) and selective plane illumination microscopy (SPIM). The main advantage of studying hair-cell regeneration in the lateral line is that it occurs throughout the life of the animal, which allows its study in the most natural context. We detail protocols to achieve continuous videomicroscopy for up to 68 hours, enabling direct observation of cellular behavior, which can provide a sensitive assay for the quantitative classification of cellular phenotypes and cell-lineage reconstruction. Modifications to this protocol should facilitate pharmacogenetic assays to identify or validate otoprotective or reparative drugs for future clinical strategies aimed at preserving aural function in humans.
• dc.format.mimetype application/pdf
• dc.identifier.citation Pinto-Teixeira F, Muzzopappa M, Swoger J, Mineo A, Sharpe J, López-Schier H. Intravital imaging of hair-cell development and regeneration in the zebrafish. Front Neuroanat. 2013 Oct 11;7:33. DOI: 10.3389/fnana.2013.00033
• dc.identifier.doi http://dx.doi.org/10.3389/fnana.2013.00033
• dc.identifier.issn 1662-5129
• dc.identifier.uri http://hdl.handle.net/10230/68307
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.ispartof Frontiers in Neuroanatomy. 2013 Oct 11;7:33
• dc.rights © 2013 Pinto-Teixeira, Muzzopappa, Swoger, Mineo, Sharpe and López-Schier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/3.0/
• dc.subject.keyword Hair cells
• dc.subject.keyword Auditory
• dc.subject.keyword Zebrafish
• dc.subject.keyword Intravital fluorescence microscopy
• dc.subject.keyword Regeneration
• dc.subject.keyword Lateral line system
• dc.subject.keyword Development
• dc.title Intravital imaging of hair-cell development and regeneration in the zebrafish
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion