A Transcript-Specific eIF3 Complex Mediates Global Translational Control of Energy Metabolism

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• dc.contributor.author Shah, Meeraca
• dc.contributor.author Su, Danca
• dc.contributor.author Scheliga, Judith S.ca
• dc.contributor.author Pluskal, Tomášca
• dc.contributor.author Boronat i Llop, Susanna, 1965-ca
• dc.contributor.author Motamedchaboki, Khaterehca
• dc.contributor.author Rosa Campos, Alexandreca
• dc.contributor.author Qi, Fengca
• dc.contributor.author Hidalgo Hernando, Elenaca
• dc.contributor.author Yanagida, Mitsuhiroca
• dc.contributor.author Wolf, Dieter A.ca
• dc.date.accessioned 2017-03-14T14:30:01Z
• dc.date.available 2017-03-14T14:30:01Z
• dc.date.issued 2016
• dc.description.abstract The multi-subunit eukaryotic translation initiation factor eIF3 is thought to assist in the recruitment of ribosomes to mRNA. The expression of eIF3 subunits is frequently disrupted in human cancers, but the specific roles of individual subunits in mRNA translation and cancer remain elusive. Using global transcriptomic, proteomic, and metabolomic profiling, we found a striking failure of Schizosaccharomyces pombe cells lacking eIF3e and eIF3d to synthesize components of the mitochondrial electron transport chain, leading to a defect in respiration, endogenous oxidative stress, and premature aging. Energy balance was maintained, however, by a switch to glycolysis with increased glucose uptake, upregulation of glycolytic enzymes, and strict dependence on a fermentable carbon source. This metabolic regulatory function appears to be conserved in human cells where eIF3e binds metabolic mRNAs and promotes their translation. Thus, via its eIF3d-eIF3e module, eIF3 orchestrates an mRNA-specific translational mechanism controlling energy metabolism that may be disrupted in cancer.
• dc.description.sponsorship This work was supported by Grant GM105802 (to D.A.W.). Parts of this work were funded by P30 Grants CA030199 and GM085764. D.A.W. is a scholar of the Foreign 1000 Talent Program funded by the Government of the People’s Republic of China.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Shah M, Su D, Scheliga JS, Pluskal T, Boronat i Llop S, Motamedchaboki K et al. A Transcript-Specific eIF3 Complex Mediates Global Translational Control of Energy Metabolism. Cell Reports. 2016;16(7):1891-902. DOI: 10.1016/j.celrep.2016.07.006
• dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2016.07.006
• dc.identifier.issn 2211-1247
• dc.identifier.uri http://hdl.handle.net/10230/28228
• dc.language.iso eng
• dc.publisher Elsevierca
• dc.relation.ispartof Cell Reports. 2016;16(7):1891-902
• dc.rights © Elsevier http://dx.doi.org/10.1016/j.celrep.2016.07.006. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.other Metabolisme energètic
• dc.title A Transcript-Specific eIF3 Complex Mediates Global Translational Control of Energy Metabolismca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion