Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri

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• dc.contributor.author Herman, Emily K.
• dc.contributor.author Najle, Sebastián R.
• dc.contributor.author Dacks, Joel B.
• dc.date.accessioned 2021-11-30T07:16:50Z
• dc.date.available 2021-11-30T07:16:50Z
• dc.date.issued 2021
• dc.description.abstract Background: The opportunistic pathogen Naegleria fowleri establishes infection in the human brain, killing almost invariably within 2 weeks. The amoeba performs piece-meal ingestion, or trogocytosis, of brain material causing direct tissue damage and massive inflammation. The cellular basis distinguishing N. fowleri from other Naegleria species, which are all non-pathogenic, is not known. Yet, with the geographic range of N. fowleri advancing, potentially due to climate change, understanding how this pathogen invades and kills is both important and timely. Results: Here, we report an -omics approach to understanding N. fowleri biology and infection at the system level. We sequenced two new strains of N. fowleri and performed a transcriptomic analysis of low- versus high-pathogenicity N. fowleri cultured in a mouse infection model. Comparative analysis provides an in-depth assessment of encoded protein complement between strains, finding high conservation. Molecular evolutionary analyses of multiple diverse cellular systems demonstrate that the N. fowleri genome encodes a similarly complete cellular repertoire to that found in free-living N. gruberi. From transcriptomics, neither stress responses nor traits conferred from lateral gene transfer are suggested as critical for pathogenicity. By contrast, cellular systems such as proteases, lysosomal machinery, and motility, together with metabolic reprogramming and novel N. fowleri proteins, are all implicated in facilitating pathogenicity within the host. Upregulation in mouse-passaged N. fowleri of genes associated with glutamate metabolism and ammonia transport suggests adaptation to available carbon sources in the central nervous system. Conclusions: In-depth analysis of Naegleria genomes and transcriptomes provides a model of cellular systems involved in opportunistic pathogenicity, uncovering new angles to understanding the biology of a rare but highly fatal pathogen.
• dc.format.mimetype application/pdf
• dc.identifier.citation Herman EK, Greninger A, van der Giezen M, Ginger ML, Ramirez-Macias I, Miller HC et al. Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri. BMC Biol. 2021;19(1):142. DOI: 10.1186/s12915-021-01078-1
• dc.identifier.doi http://dx.doi.org/10.1186/s12915-021-01078-1
• dc.identifier.issn 1741-7007
• dc.identifier.uri http://hdl.handle.net/10230/49089
• dc.language.iso eng
• dc.publisher BioMed Central
• dc.relation.ispartof BMC Biol. 2021;19(1):142
• dc.rights © The Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cytoskeleton
• dc.subject.keyword Genome sequence
• dc.subject.keyword Illumina
• dc.subject.keyword Inter-strain diversity
• dc.subject.keyword Lysosomal
• dc.subject.keyword Metabolism
• dc.subject.keyword Neuropathogenic
• dc.subject.keyword Protease
• dc.subject.keyword RNA-Seq
• dc.title Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion