CRISPR-based gene expression control for synthetic gene circuits

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• dc.contributor.author Santos Moreno, Javier
• dc.contributor.author Schaerli, Yolanda
• dc.date.accessioned 2025-07-11T11:26:18Z
• dc.date.available 2025-07-11T11:26:18Z
• dc.date.issued 2020
• dc.description.abstract Synthetic gene circuits allow us to govern cell behavior in a programmable manner, which is central to almost any application aiming to harness engineered living cells for user-defined tasks. Transcription factors (TFs) constitute the 'classic' tool for synthetic circuit construction but some of their inherent constraints, such as insufficient modularity, orthogonality and programmability, limit progress in such forward-engineering endeavors. Here we review how CRISPR (clustered regularly interspaced short palindromic repeats) technology offers new and powerful possibilities for synthetic circuit design. CRISPR systems offer superior characteristics over TFs in many aspects relevant to a modular, predictable and standardized circuit design. Thus, the choice of CRISPR technology as a framework for synthetic circuit design constitutes a valid alternative to complement or replace TFs in synthetic circuits and promises the realization of more ambitious designs.
• dc.format.mimetype application/pdf
• dc.identifier.citation Santos-Moreno J, Schaerli Y. CRISPR-based gene expression control for synthetic gene circuits. Biochem Soc Trans. 2020 Oct 30;48(5):1979-93. DOI: 10.1042/BST20200020
• dc.identifier.doi http://dx.doi.org/10.1042/BST20200020
• dc.identifier.issn 0300-5127
• dc.identifier.uri http://hdl.handle.net/10230/70897
• dc.language.iso eng
• dc.publisher Portland Press
• dc.relation.ispartof Biochem Soc Trans. 2020 Oct 30;48(5):1979-93
• dc.rights © 2020 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword CRISPR
• dc.subject.keyword Gene expression and regulation
• dc.subject.keyword Synthetic biological circuits
• dc.subject.keyword Synthetic biology
• dc.title CRISPR-based gene expression control for synthetic gene circuits
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion