Dealing with the genetic load in bacterial synthetic biology circuits: convergences with the Ohm's law

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• dc.contributor.author Carbonell Ballestero, Max, 1988-ca
• dc.contributor.author García Ramallo, Evaca
• dc.contributor.author Montañez, Raúlca
• dc.contributor.author Rodríguez Caso, Carlosca
• dc.contributor.author Macía, Javierca
• dc.date.accessioned 2016-01-20T14:55:41Z
• dc.date.available 2016-01-20T14:55:41Z
• dc.date.issued 2016
• dc.description.abstract Synthetic biology seeks to envision living cells as a matter of engineering. However, increasing evidence suggests that the genetic load imposed by the incorporation of synthetic devices in a living organism introduces a sort of unpredictability in the design process. As a result, individual part characterization is not enough to predict the behavior of designed circuits and thus, a costly trial-error process is eventually required. In this work, we provide a new theoretical framework for the predictive treatment of the genetic load. We mathematically and experimentally demonstrate that dependences among genes follow a quantitatively predictable behavior. Our theory predicts the observed reduction of the expression of a given synthetic gene when an extra genetic load is introduced in the circuit. The theory also explains that such dependence qualitatively differs when the extra load is added either by transcriptional or translational modifications. We finally show that the limitation of the cellular resources for gene expression leads to a mathematical formulation that converges to an expression analogous to the Ohm's law for electric circuits. Similitudes and divergences with this law are outlined. Our work provides a suitable framework with predictive character for the design process of complex genetic devices in synthetic biology.ca
• dc.description.sponsorship Fundación Botín, Banco de Santander through its Santander Universities Global Division [BES-2010-038940]; ERC SYNCOM [291294]; Spanish Ministry of Economy and Competitiveness [MINECO SAF2014-59284-Rand FEDER]. Funding for open access charge: Spanish Ministry of Economy and Competitiveness [MINECO SAF2014-59284-R and FEDER].
• dc.format.mimetype application/pdfca
• dc.identifier.citation Carbonell-Ballestero M, Garcia-Ramallo E, Montañez R, Rodriguez-Caso C, Macía J. Dealing with the genetic load in bacterial synthetic biology circuits: convergences with the Ohm's law. Nucleic Acids Res. 2016;44(1):496-507. DOI: 10.1093/nar/gkv1280ca
• dc.identifier.doi http://dx.doi.org/10.1093/nar/gkv1280
• dc.identifier.issn 0305-1048
• dc.identifier.uri http://hdl.handle.net/10230/25607
• dc.language.iso engca
• dc.publisher Oxford University Pressca
• dc.relation.ispartof Nucleic acids research. 2016;44(1):496-507
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BES2010-038940
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2014-59284
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/291294
• dc.rights © 2016 Carbonell-Ballestero et al. Published by Oxford University Press. This is an Open Access article distributed under the terms a Creative Commons Attribution License which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/ca
• dc.subject.other Biologia sintèticaca
• dc.subject.other Genètica bacterianaca
• dc.title Dealing with the genetic load in bacterial synthetic biology circuits: convergences with the Ohm's lawca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca