SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Mighell, Taylor L.
• dc.contributor.author Toledano, Ignasi
• dc.contributor.author Lehner, Ben, 1978-
• dc.date.accessioned 2023-11-03T07:36:35Z
• dc.date.available 2023-11-03T07:36:35Z
• dc.date.issued 2023
• dc.description.abstract Multiplexed assays of variant effects (MAVEs) have made possible the functional assessment of all possible mutations to genes and regulatory sequences. A core pillar of the approach is generation of variant libraries, but current methods are either difficult to scale or not uniform enough to enable MAVEs at the scale of gene families or beyond. We present an improved method called Scalable and Uniform Nicking (SUNi) mutagenesis that combines massive scalability with high uniformity to enable cost-effective MAVEs of gene families and eventually genomes.
• dc.description.sponsorship This work was funded by a European Research Council (ERC) Advanced grant (883742), the Spanish Ministry of Science and Innovation (PID2020-118723GB-I00, EMBL Partnership, Severo Ochoa Centre of Excellence), the la Caixa Research Foundation (LCF/PR/HR21/52410004), the AXA Research Fund, Agencia de Gestio d’Ajuts Universitaris i de Recerca (AGAUR, 2017 SGR 1322), and the CERCA Program/Generalitat de Catalunya. T.L.M. was funded by an EMBO long-term fellowship (ALTF 113-2021). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
• dc.format.mimetype application/pdf
• dc.identifier.citation Mighell TL, Toledano I, Lehner B. SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries. PLoS One. 2023 Jul 7;18(7):e0288158. DOI: 10.1371/journal.pone.0288158
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0288158
• dc.identifier.issn 1932-6203
• dc.identifier.uri http://hdl.handle.net/10230/58204
• dc.language.iso eng
• dc.publisher Public Library of Science (PLoS)
• dc.relation.ispartof PLoS One. 2023 Jul 7;18(7):e0288158
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/883742
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-118723GB-I00
• dc.rights © 2023 Mighell et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Mutagenesis
• dc.subject.keyword Library screening
• dc.subject.keyword Polymerase chain reaction
• dc.subject.keyword DNA synthesis
• dc.subject.keyword Nucleotides
• dc.subject.keyword Oligonucleotides
• dc.subject.keyword Mutation
• dc.subject.keyword Plasmid construction
• dc.title SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion