Basal association of a transcription factor favors early gene expression
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- dc.contributor.author Pinheiro, Sandrine
- dc.contributor.author Nadal Ribelles, Mariona, 1984-
- dc.contributor.author Solé, Carme
- dc.contributor.author Vincenzetti, Vincent
- dc.contributor.author Dusserre, Yves
- dc.contributor.author Posas Garriga, Francesc
- dc.contributor.author Pelet, Serge
- dc.date.accessioned 2025-07-23T07:11:51Z
- dc.date.available 2025-07-23T07:11:51Z
- dc.date.issued 2025
- dc.description.abstract Responses to extracellular signals via Mitogen-Activated Protein Kinase (MAPK) pathways control complex transcriptional programs where hundreds of genes are induced at a desired level with a specific timing. Gene expression regulation is largely encoded in the promoter of the gene, which harbors numerous transcription factor binding sites. In the mating MAPK pathway of Saccharomyces cerevisiae, one major transcription factor, Ste12, controls the chronology of gene expression necessary for the fusion of two haploid cells. Because endogenous promoters encode a large diversity of Ste12 binding sites (PRE), we engineered synthetic promoters to decipher the rules that dictate mating gene induction. Conformations of PRE dimers that allow efficient gene expression were identified. The strength of binding of Ste12 to the PRE and the distance of the binding sites to the core promoter modulate the level of induction. The speed of activation is ensured by favoring a basal association of Ste12 by using a strong dimer of PRE located in a nucleosome depleted region.
- dc.description.sponsorship Work in the Pelet lab is funded by the Swiss National Science Foundation (SNSF, 31003A_182431 and 320030-231544) and the University of Lausanne. This work in FP’s lab was founded by PID2021-124723NB-C21 from MICIU/AEI /10.13039/501100011033 and ERDF/EU and from the Ministry of Science, Innovation and Universities through the Centres of Excellence Severo Ochoa Award. FP is a recipient of an ICREA Acadèmia award (Government of Catalonia, 2024 ICREA 00084). The Ramon y Cajal Program (Spanish Ministry of Science, RYC2021-033520-I) awarded to MNR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- dc.format.mimetype application/pdf
- dc.identifier.citation Pinheiro S, Nadal-Ribelles M, Solé C, Vincenzetti V, Dusserre Y, Posas F, et al. Basal association of a transcription factor favors early gene expression. PLoS Genet. 2025 Jun 16;21(6):e1011710. DOI: 10.1371/journal.pgen.1011710
- dc.identifier.doi http://dx.doi.org/10.1371/journal.pgen.1011710
- dc.identifier.issn 1553-7390
- dc.identifier.uri http://hdl.handle.net/10230/70978
- dc.language.iso eng
- dc.publisher Public Library of Science (PLoS)
- dc.relation.ispartof PLoS Genet. 2025 Jun 16;21(6):e1011710
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-124723NB-C21
- dc.rights © 2025 Pinheiro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Nucleosomes
- dc.subject.keyword Synthetic promoters
- dc.subject.keyword Gene expression
- dc.subject.keyword Transcription factors
- dc.subject.keyword DNA transcription
- dc.subject.keyword Pheromones
- dc.subject.keyword MAPK signaling cascades
- dc.subject.keyword Transcriptional control
- dc.title Basal association of a transcription factor favors early gene expression
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion