Analysis of ECs and related compounds in plasma: artifactual isomerization and ex vivo enzymatic generation of 2-MGs
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- dc.contributor.author Pastor, Antoni
- dc.contributor.author Farré, Magí
- dc.contributor.author Fitó Colomer, Montserrat
- dc.contributor.author Fernandez-Aranda, Fernando
- dc.contributor.author de la Torre, Rafael
- dc.date.accessioned 2024-10-21T06:32:09Z
- dc.date.available 2024-10-21T06:32:09Z
- dc.date.issued 2014
- dc.description.abstract The analysis of peripheral endocannabinoids (ECs) is a good biomarker of the EC system. Their concentrations, from clinical studies, strongly depend on sample collection and time processing conditions taking place in clinical and laboratory settings. The analysis of 2-monoacylglycerols (MGs) (i.e., 2-arachidonoylglycerol or 2-oleoylglycerol) is a particularly challenging issue because of their ex vivo formation and chemical isomerization that occur after blood sample collection. We provide evidence that their ex vivo formation can be minimized by adding Orlistat, an enzymatic lipase inhibitor, to plasma. Taking into consideration the low cost of Orlistat, we recommend its addition to plasma collecting tubes while maintaining sample cold chain until storage. We have validated a method for the determination of the EC profile of a range of MGs and N-acylethanolamides in plasma that preserves the original isomer ratio of MGs. Nevertheless, the chemical isomerization of 2-MGs can only be avoided by an immediate processing and analysis of samples due to their instability during conservation. We believe that this new methodology can aid in the harmonization of the measurement of ECs and related compounds in clinical samples.
- dc.format.mimetype application/pdf
- dc.identifier.citation Pastor A, Farré M, Fitó M, Fernandez-Aranda F, de la Torre R. Analysis of ECs and related compounds in plasma: artifactual isomerization and ex vivo enzymatic generation of 2-MGs. J Lipid Res. 2014 May;55(5):966-77. DOI: 10.1194/jlr.D043794
- dc.identifier.doi http://dx.doi.org/10.1194/jlr.D043794
- dc.identifier.issn 0022-2275
- dc.identifier.uri http://hdl.handle.net/10230/68262
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Journal of Lipid Research. 2014 May;55(5):966-77
- dc.rights This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword 2-arachidonoylglycerol
- dc.subject.keyword 2-oleoylglycerol
- dc.subject.keyword Validation
- dc.subject.keyword Orlistat
- dc.subject.keyword Human
- dc.subject.keyword Endocannabinoids
- dc.subject.keyword 2-monoacylglycerol
- dc.title Analysis of ECs and related compounds in plasma: artifactual isomerization and ex vivo enzymatic generation of 2-MGs
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion