Heterologous erythromycin production across strain and plasmid construction

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• dc.contributor.author Fang, Lei
• dc.contributor.author Güell Cargol, Marc, 1982-
• dc.contributor.author Church, George M.
• dc.contributor.author Pfeifer, Blaine A.
• dc.date.accessioned 2023-04-21T06:15:27Z
• dc.date.available 2023-04-21T06:15:27Z
• dc.date.issued 2018
• dc.description.abstract The establishment of erythromycin production within the heterologous host E. coli marked an accomplishment in genetic transfer capacity. Namely, over 20 genes and 50 kb of DNA was introduced to E. coli for successful heterologous biosynthetic reconstitution. However, the prospect for production levels that approach those of the native host requires the application of engineering tools associated with E. coli. In this report, metabolic and genomic engineering were implemented to improve the E. coli cellular background and the plasmid platform supporting heterologous erythromycin formation. Results include improved plasmid stability and metabolic support for biosynthetic product formation. Specifically, the new plasmid design for erythromycin formation allowed for ≥89% stability relative to current standards (20% stability). In addition, the new strain (termed LF01) designed to improve carbon flow to the erythromycin biosynthetic pathway provided a 400% improvement in titer level. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:271-276, 2018.
• dc.format.mimetype application/pdf
• dc.identifier.citation Fang L, Guell M, Church GM, Pfeifer BA. Heterologous erythromycin production across strain and plasmid construction. Biotechnol Prog. 2018 Jan;34(1):271-6. DOI: 10.1002/btpr.2567
• dc.identifier.doi http://dx.doi.org/10.1002/btpr.2567
• dc.identifier.issn 8756-7938
• dc.identifier.uri http://hdl.handle.net/10230/56534
• dc.language.iso eng
• dc.publisher Wiley
• dc.relation.ispartof Biotechnol Prog. 2018 Jan;34(1):271-6
• dc.rights This is the peer reviewed version of the following article: Fang L, Guell M, Church GM, Pfeifer BA. Heterologous erythromycin production across strain and plasmid construction. Biotechnol Prog. 2018 Jan;34(1):271-6. DOI: 10.1002/btpr.2567, which has been published in final form at http://dx.doi.org/10.1002/btpr.2567. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword E. coli
• dc.subject.keyword Antibiotic
• dc.subject.keyword Erythromycin
• dc.subject.keyword Heterologous
• dc.subject.keyword Polyketide
• dc.title Heterologous erythromycin production across strain and plasmid construction
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion