Plasma MicroRNA profiling of Plasmodium falciparum biomass and association with severity of malaria disease

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• dc.contributor.author Gupta, Himanshu
• dc.contributor.author Rubio, Mercedes
• dc.contributor.author Sitoe, Antonio
• dc.contributor.author Varo, Rosauro
• dc.contributor.author Cisteró, Pau
• dc.contributor.author Madrid, Lola
• dc.contributor.author Cuamba, Inocencia
• dc.contributor.author Jiménez, Alfons
• dc.contributor.author Martiáñez-Vendrell, Xavier
• dc.contributor.author Barrios, Diana
• dc.contributor.author Pantano Rubiño, Lorena, 1982-
• dc.contributor.author Brimacombe, Allison
• dc.contributor.author Bustamante Pineda, Mariona
• dc.contributor.author Bassat, Quique
• dc.contributor.author Mayor, Alfredo
• dc.date.accessioned 2022-06-09T06:04:02Z
• dc.date.available 2022-06-09T06:04:02Z
• dc.date.issued 2021
• dc.description.abstract Severe malaria (SM) is a major public health problem in malaria-endemic countries. Sequestration of Plasmodium falciparum-infected erythrocytes in vital organs and the associated inflammation leads to organ dysfunction. MicroRNAs (miRNAs), which are rapidly released from damaged tissues into the host fluids, constitute a promising biomarker for the prognosis of SM. We applied next-generation sequencing to evaluate the differential expression of miRNAs in SM and in uncomplicated malaria (UM) in children in Mozambique. Six miRNAs were associated with in vitro P. falciparum cytoadhesion, severity in children, and P. falciparum biomass. Relative expression of hsa-miR-4497 quantified by TaqMan-quantitative reverse transcription PCR was higher in plasma of children with SM than those with UM (p<0.048) and again correlated with P. falciparum biomass (p = 0.033). These findings suggest that different physiopathological processes in SM and UM lead to differential expression of miRNAs and suggest a pathway for assessing their prognostic value malaria.
• dc.format.mimetype application/pdf
• dc.identifier.citation Gupta H, Rubio M, Sitoe A, Varo R, Cisteró P, Madrid L, Cuamba I, Jimenez A, Martiáñez-Vendrell X, Barrios D, Pantano L, Brimacombe A, Bustamante M, Bassat Q, Mayor A. Plasma MicroRNA profiling of Plasmodium falciparum biomass and association with severity of malaria disease. Infect Dis. 2021 Feb;27(2):430-42. DOI: 10.3201/eid2702.191795
• dc.identifier.doi http://dx.doi.org/10.3201/eid2702.191795
• dc.identifier.issn 1080-6040
• dc.identifier.uri http://hdl.handle.net/10230/53418
• dc.language.iso eng
• dc.publisher Centers for Disease Control and Prevention
• dc.relation.ispartof Infect Dis. 2021 Feb;27(2):430-42
• dc.rights This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Mozambique
• dc.subject.keyword Plasmodium falciparum
• dc.subject.keyword Spain
• dc.subject.keyword Biomarkers
• dc.subject.keyword Histidine-rich protein 2
• dc.subject.keyword Malaria
• dc.subject.keyword miRNA
• dc.subject.keyword Next-generation sequencing
• dc.subject.keyword Parasites
• dc.subject.keyword Severe malaria
• dc.subject.keyword Vector-borne infections
• dc.title Plasma MicroRNA profiling of Plasmodium falciparum biomass and association with severity of malaria disease
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion