Slow-growing cells within isogenic populations have increased RNA polymerase error rates and DNA damage

Dijk, David van; Dhar, Riddhiman; Missarova, Alsu, 1990-; Espinar, Lorena; Blevins, William Robert, 1987-; Lehner, Ben, 1978-; Carey, Lucas, 1980-

doi:http://dx.doi.org/10.1038/ncomms8972

Slow-growing cells within isogenic populations have increased RNA polymerase error rates and DNA damage

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van Dijk D, Dhar R, Missarova AM, Espinar L, Blevins WR, Lehner B et al. Slow-growing cells within isogenic populations have increased RNA polymerase error rates and DNA damage. Nature communications. 2015;6:7972. DOI: 10.1038/ncomms8972

Abstract

Isogenic cells show a large degree of variability in growth rate, even when cultured in the same environment. Such cell-to-cell variability in growth can alter sensitivity to antibiotics, chemotherapy and environmental stress. To characterize transcriptional differences associated with this variability, we have developed a method--FitFlow--that enables the sorting of subpopulations by growth rate. The slow-growing subpopulation shows a transcriptional stress response, but, more surprisingly, these cells have reduced RNA polymerase fidelity and exhibit a DNA damage response. As DNA damage is often caused by oxidative stress, we test the addition of an antioxidant, and find that it reduces the size of the slow-growing population. More generally, we find a significantly altered transcriptome in the slow-growing subpopulation that only partially resembles that of cells growing slowly due to environmental and culture conditions. Slow-growing cells upregulate transposons and express more chromosomal, viral and plasmid-borne transcripts, and thus explore a larger genotypic--and so phenotypic--space.