Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase

Ramírez-Estrada, Karla; Altabella, Teresa; Onrubia Ibáñez, Miriam, 1982-; Moyano Claramunt, Elisabet; Notredame, Cedric; Osuna, Lidia; Vanden Bossche, Robin; Goossens, Alain; Cusidó Vidal, Rosa M.; Palazón Barandela, Javier

Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase

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dc.contributor.author Ramírez-Estrada, Karlaca
dc.contributor.author Altabella, Teresaca
dc.contributor.author Onrubia Ibáñez, Miriam, 1982-ca
dc.contributor.author Moyano Claramunt, Elisabetca
dc.contributor.author Notredame, Cedricca
dc.contributor.author Osuna, Lidiaca
dc.contributor.author Vanden Bossche, Robinca
dc.contributor.author Goossens, Alainca
dc.contributor.author Cusidó Vidal, Rosa M.ca
dc.contributor.author Palazón Barandela, Javierca
dc.date.accessioned 2017-01-26T13:35:01Z
dc.date.available 2017-01-26T13:35:01Z
dc.date.issued 2016ca
dc.description.abstract Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert β-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. β-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.
dc.description.sponsorship This research has been supported by two grants from the Spanish MINECO (BIO2011-29856-C02-01 and AGL2013-43522-R) and a grant from Generalitat de Catalunya (2014SGR1434).
dc.format.mimetype application/pdfca
dc.identifier.citation Ramírez-Estrada K, Altabella T, Onrubia Ibáñez M, Moyano Claramunt E, Notredame C, Osuna L, Vanden Bossche R et al. Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase. Plant Biotechnology Journal. 2016;14(1):85-96. DOI: 10.1111/pbi.12359ca
dc.identifier.doi http://dx.doi.org/10.1111/pbi.12359
dc.identifier.issn 1467-7644ca
dc.identifier.uri http://hdl.handle.net/10230/27997
dc.language.iso engca
dc.publisher Wiley
dc.relation.ispartof Plant Biotechnology Journal. 2016;14(1):85-96
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2011-29856-C02-01
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2013-43522-R
dc.rights © Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. All articles accepted from 1st January 2016 (date of flip) are published under the terms of the Creative Commons Attribution License. Articles accepted before this date were published under the agreement as stated in the final article.ca
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri https://creativecommons.org/licenses/by/3.0/
dc.subject.keyword CDNA-AFLP
dc.subject.keyword CoA ligase
dc.subject.keyword Methyl jasmonate
dc.subject.keyword Beta-phenylalanine
dc.subject.keyword Taxol
dc.subject.keyword Taxus baccata
dc.subject.keyword Cell cultures
dc.title Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligaseca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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