H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift

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• dc.contributor.author Di Nisio, Elena
• dc.contributor.author Danovska, Svetlana
• dc.contributor.author Condemi, Livia
• dc.contributor.author Cirigliano, Angela
• dc.contributor.author Rinaldi, Teresa
• dc.contributor.author Licursi, Valerio
• dc.contributor.author Negri, Rodolfo
• dc.date.accessioned 2023-06-23T06:34:45Z
• dc.date.available 2023-06-23T06:34:45Z
• dc.date.issued 2023
• dc.description.abstract We show that in S. cerevisiae the metabolic diauxic shift is associated with a H3 lysine 4 tri-methylation (H3K4me3) increase which involves a significant fraction of transcriptionally induced genes which are required for the metabolic changes, suggesting a role for histone methylation in their transcriptional regulation. We show that histone H3K4me3 around the start site correlates with transcriptional induction in some of these genes. Among the methylation-induced genes are IDP2 and ODC1, which regulate the nuclear availability of α-ketoglutarate, which, as a cofactor for Jhd2 demethylase, regulates H3K4 tri-methylation. We propose that this feedback circuit could be used to regulate the nuclear α-ketoglutarate pool concentration. We also show that yeast cells adapt to the absence of Jhd2 by decreasing Set1 methylation activity.
• dc.format.mimetype application/pdf
• dc.identifier.citation Di Nisio E, Danovska S, Condemi L, Cirigliano A, Rinaldi T, Licursi V, Negri R. H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift. Metabolites. 2023 Mar 31;13(4):507. DOI: 10.3390/metabo13040507
• dc.identifier.doi http://dx.doi.org/10.3390/metabo13040507
• dc.identifier.issn 2218-1989
• dc.identifier.uri http://hdl.handle.net/10230/57315
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Metabolites. 2023 Mar 31;13(4):507
• dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword H3K4 tri-methylation
• dc.subject.keyword Diauxic shift
• dc.subject.keyword Transcriptional regulation
• dc.title H3 lysine 4 methylation is required for full activation of genes involved in α-ketoglutarate availability in the nucleus of yeast cells after diauxic shift
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion