A Single Amino Acid Deletion (ΔF1502) in the S6 Segment of CaV2.1 Domain III Associated with Congenital Ataxia Increases Channel Activity and Promotes Ca2+ Influx

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• dc.contributor.author Bahamonde Santos, María Isabel, 1972-ca
• dc.contributor.author Serra Pascual, Selma A., 1981-ca
• dc.contributor.author Drechsel, Oliverca
• dc.contributor.author Rahman, Rubayteca
• dc.contributor.author Marcé-Grau, Annaca
• dc.contributor.author Prieto, Martaca
• dc.contributor.author Ossowski, Stephanca
• dc.contributor.author Macaya, Alfonsca
• dc.contributor.author Fernández-Fernández, José Manuel, 1967-ca
• dc.date.accessioned 2017-03-08T13:03:51Z
• dc.date.available 2017-03-08T13:03:51Z
• dc.date.issued 2015
• dc.description.abstract Mutations in the CACNA1A gene, encoding the pore-forming CaV2.1 (P/Q-type) channel α1A subunit, result in heterogeneous human neurological disorders, including familial and sporadic hemiplegic migraine along with episodic and progressive forms of ataxia. Hemiplegic Migraine (HM) mutations induce gain-of-channel function, mainly by shifting channel activation to lower voltages, whereas ataxia mutations mostly produce loss-of-channel function. However, some HM-linked gain-of-function mutations are also associated to congenital ataxia and/or cerebellar atrophy, including the deletion of a highly conserved phenylalanine located at the S6 pore region of α1A domain III (ΔF1502). Functional studies of ΔF1502 CaV2.1 channels, expressed in Xenopus oocytes, using the non-physiological Ba2+ as the charge carrier have only revealed discrete alterations in channel function of unclear pathophysiological relevance. Here, we report a second case of congenital ataxia linked to the ΔF1502 α1A mutation, detected by whole-exome sequencing, and analyze its functional consequences on CaV2.1 human channels heterologously expressed in mammalian tsA-201 HEK cells, using the physiological permeant ion Ca2+. ΔF1502 strongly decreases the voltage threshold for channel activation (by ~ 21 mV), allowing significantly higher Ca2+ current densities in a range of depolarized voltages with physiological relevance in neurons, even though maximal Ca2+ current density through ΔF1502 CaV2.1 channels is 60% lower than through wild-type channels. ΔF1502 accelerates activation kinetics and slows deactivation kinetics of CaV2.1 within a wide range of voltage depolarization. ΔF1502 also slowed CaV2.1 inactivation kinetic and shifted the inactivation curve to hyperpolarized potentials (by ~ 28 mV). ΔF1502 effects on CaV2.1 activation and deactivation properties seem to be of high physiological relevance. Thus, ΔF1502 strongly promotes Ca2+ influx in response to either single or trains of action potential-like waveforms of different durations. Our observations support a causative role of gain-of-function CaV2.1 mutations in congenital ataxia, a neurodevelopmental disorder at the severe-most end of CACNA1A-associated phenotypic spectrum.
• dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (SAF2012-31089 to JMF-F; SEV-2012-0208 to Centre for Genomic Regulation, “Centro de Excelencia Severo Ochoa 2013-2017”; and MDM-2014-0370 through the “María de Maeztu” Programme for Units of Excellence in R&D to “Departament de Ciències Experimentals i de la Salut”), FEDER Funds, Fondo de Investigación Sanitaria, Instituto Carlos III, Spain (RIC RD12/0042/0014, Red HERACLES, and Grant PI12/1005 to AM). AM-G is a predoctoral fellow supported by Vall d’Hebron Institut de Recerca, Barcelona, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Bahamonde Santos MI, Serra Pascual SA, Drechsel O, Rahman R, Marcé-Grau A, Prieto M et al. A Single Amino Acid Deletion (ΔF1502) in the S6 Segment of CaV2.1 Domain III Associated with Congenital Ataxia Increases Channel Activity and Promotes Ca2+ Influx. PLoS ONE. 2015;10(12):e0146035. DOI: 10.1371/journal.pone.0146035
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0146035
• dc.identifier.issn 1932-6203
• dc.identifier.uri http://hdl.handle.net/10230/28186
• dc.language.iso eng
• dc.publisher Public Library of Science (PLoS)ca
• dc.relation.ispartof PLoS ONE. 2015;10(12):e0146035
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2012-31089
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
• dc.rights © 2015 Bahamonde et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Ataxia
• dc.subject.keyword Membrane potential
• dc.subject.keyword Action potentials
• dc.subject.keyword HEK 293 cells
• dc.subject.keyword Neurons
• dc.subject.keyword Atrophy
• dc.subject.keyword Mouse models
• dc.subject.keyword Migraine
• dc.title A Single Amino Acid Deletion (ΔF1502) in the S6 Segment of CaV2.1 Domain III Associated with Congenital Ataxia Increases Channel Activity and Promotes Ca2+ Influxca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion