Differentiating functional human islet-like aggregates from pluripotent stem cells
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- dc.contributor.author Barsby, Tom
- dc.contributor.author Ibrahim, Hazem
- dc.contributor.author Lithovius, Väinö
- dc.contributor.author Montaser, Hossam
- dc.contributor.author Balboa, Diego
- dc.contributor.author Vähäkangas, Eliisa
- dc.contributor.author Chandra, Vikash
- dc.contributor.author Saarimäki-Vire, Jonna
- dc.contributor.author Otonkoski, Timo
- dc.date.accessioned 2022-11-09T07:25:17Z
- dc.date.available 2022-11-09T07:25:17Z
- dc.date.issued 2022
- dc.description.abstract We present here a robust and reliable protocol by which to differentiate pancreatic islet-like aggregates (SC-islets) from human pluripotent stem cells. The 7-stage protocol mimics developmental patterning factors that induce endocrine lineage formation and spans monolayer, microwell, and aggregate suspension culture. The SC-islets demonstrate dynamic glucose-sensitive insulin secretion and an endocrine cell composition similar to those of primary human islets. SC-islets generated using this optimized protocol are suitable for in vitro modeling of islet cell pathophysiology and therapeutic applications. For complete details on the use and execution of this protocol, please refer to Balboa et al. (2022).
- dc.format.mimetype application/pdf
- dc.identifier.citation Barsby T, Ibrahim H, Lithovius V, Montaser H, Balboa D, Vähäkangas E, Chandra V, Saarimäki-Vire J, Otonkoski T. Differentiating functional human islet-like aggregates from pluripotent stem cells. STAR Protoc. 2022 Sep 21;3(4):101711. DOI: 10.1016/j.xpro.2022.101711
- dc.identifier.doi http://dx.doi.org/10.1016/j.xpro.2022.101711
- dc.identifier.issn 2666-1667
- dc.identifier.uri http://hdl.handle.net/10230/54768
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof STAR Protoc. 2022 Sep 21;3(4):101711
- dc.rights © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.keyword Cell differentiation
- dc.subject.keyword Stem cells
- dc.title Differentiating functional human islet-like aggregates from pluripotent stem cells
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion