Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM
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- dc.contributor.author Mayer, Jürgen, 1977-
- dc.contributor.author Robert Moreno, Alexandre
- dc.contributor.author Sharpe, James
- dc.contributor.author Swoger, Jim
- dc.date.accessioned 2019-11-21T12:50:47Z
- dc.date.available 2019-11-21T12:50:47Z
- dc.date.issued 2018
- dc.description.abstract Light sheet fluorescence microscopy (LSFM) is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs. However, LSFM can suffer from optical artifacts for which there is no intrinsic solution. The attenuation of light due to absorbing material causes "shadow" artifacts along both the illumination and detection paths. Several approaches have been introduced to reduce this problem, including scanning illumination and multi-view imaging. However, neither of these approaches completely eliminates the problem. If the distribution of the absorbing material is complex, shadows cannot be avoided. We introduce a new approach that relies on multi-modal integration of two very different mesoscopic techniques. Unlike LSFM, optical projection tomography (OPT) can operate in transmission mode to create a voxel map of the 3D distribution of the sample's optical attenuation. Here, we demonstrate a hybrid instrument (OPTiSPIM) that can quantify this attenuation and use the information to correct the shadow artifacts of LSFM.
- dc.description.sponsorship The research was funded in part by the European Union’s 7th Framework VIBRANT project (No. 228933 of the FP7-NMP) and the Sinergia project (CRII3_125477) of the Swiss National Science Foundation (SNSF). We acknowledge support from the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013–2017” and from the CERCA Programme/Generalitat de Catalunya.
- dc.format.mimetype application/pdf
- dc.identifier.citation Mayer J, Robert-Moreno A, Sharpe J, Swoger J. Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM. Light Sci Appl. 2018;7:70. DOI: 10.1038/s41377-018-0068-z
- dc.identifier.doi http://dx.doi.org/10.1038/s41377-018-0068-z
- dc.identifier.issn 2047-7538
- dc.identifier.uri http://hdl.handle.net/10230/42922
- dc.language.iso eng
- dc.publisher Nature Research
- dc.relation.ispartof Light: Science & Applications. 2018;7:70
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/228933
- dc.rights © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.title Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion