Protocol to generate a patient derived xenograft model of acquired resistance to immunotherapy in humanized mice

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  • dc.contributor.author Martínez Sabadell, Alex
  • dc.contributor.author Ovejero Romero, Pablo
  • dc.contributor.author Arribas, Joaquín
  • dc.contributor.author Arenas, Enrique J.
  • dc.date.accessioned 2023-05-12T06:21:38Z
  • dc.date.available 2023-05-12T06:21:38Z
  • dc.date.issued 2022
  • dc.description.abstract Immunotherapy has revolutionized cancer treatment, but preclinical models are required to understand immunotherapy resistance mechanisms underlying patient relapse. This protocol describes how to generate an acquired resistance humanized in vivo model to immunotherapies in patient-derived xenografts (PDX). We detail steps to inject human CD34+ cells into NSG mice, followed by generation of immunoresistant PDX in humanized mice. This approach recapitulates the human immune system, allowing investigators to generate preclinical resistance models to different immunotherapies for identifying the resistant phenotype. For complete details on the use and execution of this protocol, please refer to Martínez-Sabadell et al., 2022 and Arenas et al. (2021).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Martínez-Sabadell A, Ovejero Romero P, Arribas J, Arenas EJ. Protocol to generate a patient derived xenograft model of acquired resistance to immunotherapy in humanized mice. STAR Protoc. 2022 Oct 26;3(4):101712. DOI: 10.1016/j.xpro.2022.101712
  • dc.identifier.doi http://dx.doi.org/10.1016/j.xpro.2022.101712
  • dc.identifier.issn 2666-1667
  • dc.identifier.uri http://hdl.handle.net/10230/56787
  • dc.language.iso eng
  • dc.publisher Elsevier
  • dc.relation.ispartof STAR Protoc. 2022 Oct 26;3(4):101712
  • dc.rights © 2022 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
  • dc.subject.keyword Cancer
  • dc.subject.keyword Cell isolation
  • dc.subject.keyword Health sciences
  • dc.subject.keyword Immunology
  • dc.subject.keyword Model organisms
  • dc.subject.keyword Stem cells
  • dc.title Protocol to generate a patient derived xenograft model of acquired resistance to immunotherapy in humanized mice
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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