Nail melatonin content: a suitable non-invasive marker of melatonin production
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Gómez-Gómez, Àlex
- dc.contributor.author Montero-San Martin, Blanca
- dc.contributor.author Haro, Noemí
- dc.contributor.author Pozo Mendoza, Óscar J.
- dc.date.accessioned 2021-07-22T07:11:44Z
- dc.date.available 2021-07-22T07:11:44Z
- dc.date.issued 2021
- dc.description.abstract Melatonin plays multiple physiological roles in the human body. Evaluation of melatonin production by the determination of urinary 6-sulfatoxymelatonin in 24-h samples has important drawbacks which hinder the successful evaluation of melatonin production in large cohorts. Here, we evaluated the potential of nail analysis for estimating melatonin production. Firstly, mass spectrometry methodology for the determination of melatonin in nails was optimized and successfully validated. The method was found to be linear in the range 6.5-830 fg/mg with intraday and interday accuracy in the range 100-104 %, precision below 15 % and a LOD of 3.5 fg/mg. Secondly, nail melatonin concentrations from 84 volunteers (age 5-96) were determined. The expected correlation between melatonin and age was obtained (correlation coefficient -0.615; p < 0.001). Additionally, we showed that fingernails are preferable to toenails to determine nail melatonin content. Finally, fingernails collected for 180 days after melatonin administration (two volunteers, 1.9 mg/night during 5 days) were analyzed. Nail melatonin concentrations immediately rose after administration and went back to pre-administration values after ≈100 days in both volunteers. Our results suggest that melatonin determination in nails is a suitable non-invasive tool for the estimation of global melatonin production. Due to the easy collection and storage of nails, the long-term information obtained and the multiple functions of melatonin, nail melatonin content might complement dim light melatonin onset, which is commonly measured from plasma/saliva samples, paving the way for melatonin research.
- dc.format.mimetype application/pdf
- dc.identifier.citation Gómez-Gómez A, Montero-San-Martin B, Haro N, Pozo OJ. Nail melatonin content: a suitable non-invasive marker of melatonin production. Int J Mol Sci. 2021 Jan 18; 22(2): 921. DOI: 10.3390/ijms22020921
- dc.identifier.doi http://dx.doi.org/10.3390/ijms22020921
- dc.identifier.issn 1422-0067
- dc.identifier.uri http://hdl.handle.net/10230/48274
- dc.language.iso eng
- dc.publisher MDPI
- dc.rights Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Aging
- dc.subject.keyword Fingernails
- dc.subject.keyword Liquid chromatography
- dc.subject.keyword Mass spectrometry
- dc.subject.keyword Melatonin
- dc.subject.keyword Nails
- dc.title Nail melatonin content: a suitable non-invasive marker of melatonin production
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion