Synthetic lateral inhibition in periodic pattern forming microbial colonies

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• dc.contributor.author Duran Nebreda, Salvador, 1987-
• dc.contributor.author Pla, Jordi
• dc.contributor.author Vidiella Rocamora, Blai
• dc.contributor.author Piñero, Jordi
• dc.contributor.author Conde Pueyo, Núria, 1983-
• dc.contributor.author Solé Vicente, Ricard, 1962-
• dc.date.accessioned 2022-01-25T07:29:35Z
• dc.date.available 2022-01-25T07:29:35Z
• dc.date.issued 2021
• dc.description.abstract Multicellular entities are characterized by intricate spatial patterns, intimately related to the functions they perform. These patterns are often created from isotropic embryonic structures, without external information cues guiding the symmetry breaking process. Mature biological structures also display characteristic scales with repeating distributions of signals or chemical species across space. Many candidate patterning modules have been used to explain processes during development and typically include a set of interacting and diffusing chemicals or agents known as morphogens. Great effort has been put forward to better understand the conditions in which pattern-forming processes can occur in the biological domain. However, evidence and practical knowledge allowing us to engineer symmetry-breaking is still lacking. Here we follow a different approach by designing a synthetic gene circuit in E. coli that implements a local activation long-range inhibition mechanism. The synthetic gene network implements an artificial differentiation process that changes the physicochemical properties of the agents. Using both experimental results and modeling, we show that the proposed system is capable of symmetry-breaking leading to regular spatial patterns during colony growth. Studying how these patterns emerge is fundamental to further our understanding of the evolution of biocomplexity and the role played by self-organization. The artificial system studied here and the engineering perspective on embryogenic processes can help validate developmental theories and identify universal properties underpinning biological pattern formation, with special interest for the area of synthetic developmental biology.
• dc.description.sponsorship This study was supported by a European Research Council Advanced Grant (SYNCOM), the Botin Foundation, by Banco Santander through its Santander Universities Global Division and by a MINECO Grant FIS2015-67616-P. This work has also received support by the Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya and by the Santa Fe Institute.
• dc.format.mimetype application/pdf
• dc.identifier.citation Duran-Nebreda S, Pla J, Vidiella B, Piñero J, Conde-Pueyo N, Solé R. Synthetic lateral inhibition in periodic pattern forming microbial colonies. ACS Synth Biol. 2021;10(2):277-85. DOI: 10.1021/acssynbio.0c00318
• dc.identifier.doi http://dx.doi.org/10.1021/acssynbio.0c00318
• dc.identifier.issn 2161-5063
• dc.identifier.uri http://hdl.handle.net/10230/52302
• dc.language.iso eng
• dc.publisher American Chemical Society (ACS)
• dc.relation.ispartof ACS Synth Biol. 2021;10(2):277-85
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/FIS2015-67616-P
• dc.rights © 2021 American Chemical Society. This work is licensed under a Creative Commons Attribution 4.0 International License
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Developmental biology
• dc.subject.keyword Lateral inhibition
• dc.subject.keyword Pattern formation
• dc.subject.keyword Synthetic biology
• dc.title Synthetic lateral inhibition in periodic pattern forming microbial colonies
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion