Fiber-like action of d-fagomine on the gut microbiota and body weight of healthy rats

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• dc.contributor.author Ramos-Romero, Sara
• dc.contributor.author Ponomarenko, Julia
• dc.contributor.author Amézqueta, Susana
• dc.contributor.author Hereu, Mercè
• dc.contributor.author Miralles-Pérez, Bernat
• dc.contributor.author Romeu, Marta
• dc.contributor.author Méndez, Lucía
• dc.contributor.author Medina, Isabel
• dc.contributor.author Torres, Josep Lluís
• dc.date.accessioned 2023-01-24T07:20:20Z
• dc.date.available 2023-01-24T07:20:20Z
• dc.date.issued 2022
• dc.description.abstract The goal of this work is to explore if the changes induced by d-fagomine in the gut microbiota are compatible with its effect on body weight and inflammation markers in rats. Methods: Sprague Dawley rats were fed a standard diet supplemented with d-fagomine (or not, for comparison) for 6 months. The variables measured were body weight, plasma mediators of inflammation (hydroxyeicosatetraenoic acids, leukotriene B4, and IL-6), and the concentration of acetic acid in feces and plasma. The composition and diversities of microbiota in cecal content and feces were estimated using 16S rRNA metabarcoding and high-throughput sequencing. We found that after just 6 weeks of intake d-fagomine significantly reduced body weight gain, increased the plasma acetate concentration, and reduced the plasma concentration of the pro-inflammatory biomarkers' leukotriene B4, interleukin 6 and 12 hydroxyeicosatetraenoic acids. These changes were associated with a significantly increased prevalence of Bacteroides and Prevotella feces and increased Bacteroides, Prevotella, Clostridium, and Dysgonomonas while reducing Anaerofilum, Blautia, and Oribacterium in cecal content. In conclusion, d-fagomine induced changes in the composition and diversity of gut microbiota similar to those elicited by dietary fiber and compatible with its anti-inflammatory and body-weight-reducing effects.
• dc.description.sponsorship This work was supported by the Spanish Ministry of Economy, Industry, and Competitiveness (grant numbers AGL2017-83599-R, PID2020-117009RB-I00). We acknowledge the support of the CRG by the MEIC to the EMBL partnership, Centro de Excelencia Severo Ochoa, and CERCA Programme/Generalitat de Catalunya.
• dc.format.mimetype application/pdf
• dc.identifier.citation Ramos-Romero S, Ponomarenko J, Amézqueta S, Hereu M, Miralles-Pérez B, Romeu M, Méndez L, Medina I, Torres JL. Fiber-like action of d-fagomine on the gut microbiota and body weight of healthy rats. Nutrients. 2022 Nov 3;14(21):4656. DOI: 10.3390/nu14214656
• dc.identifier.doi http://dx.doi.org/10.3390/nu14214656
• dc.identifier.issn 2072-6643
• dc.identifier.uri http://hdl.handle.net/10230/55414
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Nutrients. 2022 Nov 3;14(21):4656
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-83599-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-117009RB-I00
• dc.rights © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Body weight
• dc.subject.keyword d-fagomine
• dc.subject.keyword Gut microbiota
• dc.subject.keyword Iminocyclitol
• dc.subject.keyword Iminosugar
• dc.subject.keyword Inflammation
• dc.title Fiber-like action of d-fagomine on the gut microbiota and body weight of healthy rats
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion