Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast

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• dc.contributor.author Bosio, Maria Cristinaca
• dc.contributor.author Fermi, Beatriceca
• dc.contributor.author Spagnoli, Gloriaca
• dc.contributor.author Levati, Elisabettaca
• dc.contributor.author Rubbi, Liudmillaca
• dc.contributor.author Ferrari, Robertoca
• dc.contributor.author Pellegrini, Matteoca
• dc.contributor.author Dieci, Giorgioca
• dc.date.accessioned 2018-06-28T07:40:23Z
• dc.date.available 2018-06-28T07:40:23Z
• dc.date.issued 2017
• dc.description.abstract Ribosome biogenesis in Saccharomyces cerevisiae involves a regulon of >200 genes (Ribi genes) coordinately regulated in response to nutrient availability and cellular growth rate. Two cis-acting elements called PAC and RRPE are known to mediate Ribi gene repression in response to nutritional downshift. Here, we show that most Ribi gene promoters also contain binding sites for one or more General Regulatory Factors (GRFs), most frequently Abf1 and Reb1, and that these factors are enriched in vivo at Ribi promoters. Abf1/Reb1/Tbf1 promoter association was required for full Ribi gene expression in rich medium and for its modulation in response to glucose starvation, characterized by a rapid drop followed by slow recovery. Such a response did not entail changes in Abf1 occupancy, but it was paralleled by a quick increase, followed by slow decrease, in Rpd3L histone deacetylase occupancy. Remarkably, Abf1 site disruption also abolished Rpd3L complex recruitment in response to starvation. Extensive mutational analysis of the DBP7 promoter revealed a complex interplay of Tbf1 sites, PAC and RRPE in the transcriptional regulation of this Ribi gene. Our observations point to GRFs as new multifaceted players in Ribi gene regulation both during exponential growth and under repressive conditions.
• dc.format.mimetype application/pdf
• dc.identifier.citation Bosio MC, Fermi B, Spagnoli G, Levati E, Rubbi L, Ferrari R et al. Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast. Nucleic Acids Res. 2017 May 5;45(8):4493-4506. DOI: 10.1093/nar/gkx058
• dc.identifier.doi http://dx.doi.org/10.1093/nar/gkx058
• dc.identifier.issn 0305-1048
• dc.identifier.uri http://hdl.handle.net/10230/34991
• dc.language.iso eng
• dc.publisher Oxford University Pressca
• dc.relation.ispartof Nucleic Acids Res. 2017 May 5;45(8):4493-4506
• dc.rights © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
• dc.subject.keyword Dead-box rna helicases
• dc.subject.keyword Dna-binding proteins
• dc.subject.keyword Gene expression regulation
• dc.subject.keyword Glucose
• dc.subject.keyword Histone deacetylase 1
• dc.subject.keyword Saccharomyces cerevisiae
• dc.subject.keyword Ribosomes
• dc.title Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeastca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion