A new method for extracting skin microbes allows metagenomic analysis of whole-deep skin
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- dc.contributor.author García Garcerà, Marcca
- dc.contributor.author Garcia Etxebarria, Koldoca
- dc.contributor.author Coscollà, Mireiaca
- dc.contributor.author Latorre, Amparoca
- dc.contributor.author Calafell i Majó, Francescca
- dc.date.accessioned 2015-05-15T07:10:20Z
- dc.date.available 2015-05-15T07:10:20Z
- dc.date.issued 2013ca
- dc.description.abstract In the last decade, an extensive effort has been made to characterize the human microbiota, due to its clinical and economic interests. However, a metagenomic approach to the skin microbiota is hampered by the high proportion of host DNA that is recovered. In contrast with the burgeoning field of gut metagenomics, skin metagenomics has been hindered by the absence of an efficient method to avoid sequencing the host DNA. We present here a method for recovering microbial DNA from skin samples, based on a combination of molecular techniques. We have applied this method to mouse skin, and have validated it by standard, quantitative PCR and amplicon sequencing of 16S rRNA. The taxonomic diversity recovered was not altered by this new method, as proved by comparing the phylogenetic structure revealed by 16S rRNA sequencing in untreated vs. treated samples. As proof of concept, we also present the first two mouse skin metagenomes, which allowed discovering new taxa (not only prokaryotes but also viruses and eukaryots) not reachable by 16S rRNA sequencing, as well as to characterize the skin microbiome functional landscape. Our method paves the way for the development of skin metagenomics, which will allow a much deeper knowledge of the skin microbiome and its relationship with the host, both in a healthy state and in relation to disease.en
- dc.description.sponsorship This work was funded by the Spanish Ministry of Science and Innovation (MICINN)[grant numbers SAF2010-16240 and BFU2009-12895-CO2-01]. MGG was supported by a predoctoral fellowship from the Spanish Ministry of Scienceand Innovation [Grant number BES-2008-006029].en
- dc.format.mimetype application/pdfca
- dc.identifier.citation García-Garcerà M, García-Etxebarria K, Coscolla M, Latorre A, Calafell F. A new method for extracting skin microbes allows metagenomic analysis of whole-deep skin. PLoS ONE. 2013;8(9):e74914. DOI: 10.1371/journal.pone.0074914ca
- dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0074914
- dc.identifier.issn 1932-6203ca
- dc.identifier.uri http://hdl.handle.net/10230/23583
- dc.language.iso engca
- dc.publisher Public Library of Science (PLoS)ca
- dc.relation.ispartof PLoS ONE. 2013;8(9):e74914
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2010-16240
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-12895
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BES2008-006029
- dc.rights © 2013 Garcia-Garcerà et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.other Pell -- Microbiologiaca
- dc.subject.other Genètica bacterianaca
- dc.subject.other Gensca
- dc.title A new method for extracting skin microbes allows metagenomic analysis of whole-deep skinen
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca