Estimation of cell proliferation dynamics using CFSE data

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• dc.contributor.author Banks, H. Thomas
• dc.contributor.author Sutton, Karyn L.
• dc.contributor.author Thompson, W. Clayton
• dc.contributor.author Bocharov, Gennady A.
• dc.contributor.author Roose, Dirk
• dc.contributor.author Schenkel, Tim
• dc.contributor.author Meyerhans, Andreas
• dc.date.accessioned 2019-02-21T08:35:19Z
• dc.date.available 2019-02-21T08:35:19Z
• dc.date.issued 2011
• dc.description.abstract Advances in fluorescent labeling of cells as measured by flow cytometry have allowed for quantitative studies of proliferating populations of cells. The investigations (Luzyanina et al. in J. Math. Biol. 54:57-89, 2007; J. Math. Biol., 2009; Theor. Biol. Med. Model. 4:1-26, 2007) contain a mathematical model with fluorescence intensity as a structure variable to describe the evolution in time of proliferating cells labeled by carboxyfluorescein succinimidyl ester (CFSE). Here, this model and several extensions/modifications are discussed. Suggestions for improvements are presented and analyzed with respect to statistical significance for better agreement between model solutions and experimental data. These investigations suggest that the new decay/label loss and time dependent effective proliferation and death rates do indeed provide improved fits of the model to data. Statistical models for the observed variability/noise in the data are discussed with implications for uncertainty quantification. The resulting new cell dynamics model should prove useful in proliferation assay tracking and modeling, with numerous applications in the biomedical sciences.
• dc.format.mimetype application/pdf
• dc.identifier.citation Banks HT, Sutton KL, Thompson WC, Bocharov G, Roose D, Schenkel T et al. Estimation of cell proliferation dynamics using CFSE data. Bull Math Biol. 2011;73(1):116-50. DOI: 10.1007/s11538-010-9524-5
• dc.identifier.doi http://dx.doi.org/10.1007/s11538-010-9524-5
• dc.identifier.issn 0092-8240
• dc.identifier.uri http://hdl.handle.net/10230/36642
• dc.language.iso eng
• dc.publisher Springer
• dc.relation.ispartof Bulletin of Mathematical Biology. 2011;73(1):116-50
• dc.rights © Springer The final publication is available at Springer via http://dx.doi.org/10.1007/s11538-010-9524-5.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Cell proliferation
• dc.subject.keyword CFSE
• dc.subject.keyword Label structured population dynamics
• dc.subject.keyword Partial differential equations
• dc.subject.keyword Inverse problems
• dc.title Estimation of cell proliferation dynamics using CFSE data
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion