Novel snail1 target proteins in human colon cancer identified by proteomic analysis

Larriba, María Jesús; Casado Vela, Juan; Pendás Franco, Natalia; Peña, Raúl; García de Herreros, Antonio; Berciano, María Teresa; Lafarga, Miguel; Casal, José Ignacio; Muñoz, Alberto

Novel snail1 target proteins in human colon cancer identified by proteomic analysis

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dc.contributor.author Larriba, María Jesúsca
dc.contributor.author Casado Vela, Juanca
dc.contributor.author Pendás Franco, Nataliaca
dc.contributor.author Peña, Raúlca
dc.contributor.author García de Herreros, Antonioca
dc.contributor.author Berciano, María Teresaca
dc.contributor.author Lafarga, Miguelca
dc.contributor.author Casal, José Ignacioca
dc.contributor.author Muñoz, Albertoca
dc.date.accessioned 2015-04-29T07:24:07Z
dc.date.available 2015-04-29T07:24:07Z
dc.date.issued 2010ca
dc.description.abstract Background: The transcription factor Snail1 induces epithelial-to-mesenchymal transition (EMT), a process responsible for the acquisition of invasiveness during tumorigenesis. Several transcriptomic studies have reported Snail1-regulated genes in different cell types, many of them involved in cell adhesion. However, only a few studies have used proteomics as a tool for the characterization of proteins mediating EMT. Methodology/Principal Findings: We identified by proteomic analysis using 2D-DIGE electrophoresis combined with MALDI-TOF-TOF and ESI-linear ion trap mass spectrometry a number of proteins with variable functions whose expression is modulated by Snail1 in SW480-ADH human colon cancer cells. Validation was performed by Western blot and immunofluorescence analyses. Snail1 repressed several members of the 14-3-3 family of phosphoserine/phosphothreonine binding proteins and also the expression of the Proliferation-associated protein 2G4 (PA2G4) that was mainly localized at the nuclear Cajal bodies. In contrast, the expression of two proteins involved in RNA processing, the Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) and the Splicing factor proline/glutamine-rich (SFPQ), was higher in Snail1-expressing cells than in controls. The regulation of 14-3-3e, 14-3-3t, 14-3-3f and PA2G4 by Snail1 was reproduced in HT29 colon cancer cells. In addition, we found an inverse correlation between 14-3-3 s and Snail1 expression in human colorectal tumors. Conclusions/Significance: We have identified a set of novel Snail1 target proteins in colon cancer that expand the cellular processes affected by Snail1 and thus its relevance for cell function and phenotype.en
dc.description.sponsorship This work was supported by the Ministerio de Ciencia e Innovación of Spain (SAF2007-60341, BIO2006-07689, ISCIII-RETIC RD06/0020/0009 and RD06/ 0020/0040), Comunidad de Madrid (S-GEN-0266/2006) and the European Union (MRTN-CT-2005-019496, NucSys). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscripten
dc.format.mimetype application/pdfca
dc.identifier.citation Larriba MJ, Casado-Vela J, Pendas-Franco N, Pena R, Herreros de AG, Berciano M et al. Novel snail1 target proteins in human colon cancer identified by proteomic analysis. PLoS ONE. 2010;5(4):e10221. DOI: 10.1371/journal.pone.0010221ca
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0010221
dc.identifier.issn 1932-6203ca
dc.identifier.uri http://hdl.handle.net/10230/23486
dc.language.iso engca
dc.publisher Public Library of Science (PLoS)ca
dc.relation.ispartof PLoS ONE. 2010;5(4):e10221
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/SAF2007-60341
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BIO2006-07689
dc.rights © 2010 Larriba et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.subject.other Proteòmicaca
dc.subject.other Mesènquimaca
dc.subject.other Cèl·lules epitelials -- Càncer -- Aspectes genèticsca
dc.title Novel snail1 target proteins in human colon cancer identified by proteomic analysisen
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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