Yeast-to-hypha transition of schizosaccharomyces japonicus in response to environmental stimuli

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• dc.contributor.author Kinnaer, Cassandre
• dc.contributor.author Dudin, Omaya
• dc.contributor.author Martin, Sophie G.
• dc.contributor.author Lew, Daniel J.
• dc.date.accessioned 2023-01-16T12:07:06Z
• dc.date.available 2023-01-16T12:07:06Z
• dc.date.issued 2019
• dc.description.abstract Many fungal species are dimorphic, exhibiting both unicellular yeast-like and filamentous forms. Schizosaccharomyces japonicus, a member of the fission yeast clade, is one such dimorphic fungus. Here, we first identify fruit extracts as natural, stress-free, starvation-independent inducers of filamentation, which we use to describe the properties of the dimorphic switch. During the yeast-to-hypha transition, the cell evolves from a bipolar to a unipolar system with 10-fold accelerated polarized growth but constant width, vacuoles segregated to the nongrowing half of the cell, and hyper-lengthening of the cell. We demonstrate unusual features of S. japonicus hyphae: these cells lack a Spitzenkörper, a vesicle distribution center at the hyphal tip, but display more rapid cytoskeleton-based transport than the yeast form, with actin cables being essential for the transition. S. japonicus hyphae also remain mononuclear and undergo complete cell divisions, which are highly asymmetric: one daughter cell inherits the vacuole, the other the growing tip. We show that these elongated cells scale their nuclear size, spindle length, and elongation rates, but display altered division size controls. This establishes S. japonicus as a unique system that switches between symmetric and asymmetric modes of growth and division.
• dc.format.mimetype application/pdf
• dc.identifier.citation Kinnaer C, Dudin O, Martin SG, Lew DJ. Yeast-to-hypha transition of schizosaccharomyces japonicus in response to environmental stimuli. MBoC. 2019 Apr 01;30(8):975-91. DOI: 10.1091/mbc.E18-12-0774
• dc.identifier.doi http://dx.doi.org/10.1091/mbc.E18-12-0774
• dc.identifier.issn 1939-4586
• dc.identifier.uri http://hdl.handle.net/10230/55282
• dc.language.iso eng
• dc.publisher American Society for Cell Biology
• dc.relation.ispartof Molecular Biology of the Cell. 2019 Apr 01;30(8):975-91
• dc.rights © 2019 Kinnaer et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/
• dc.subject.other Fongs -- Espècimens tipus
• dc.subject.other Plantes -- Cèl·lules i teixits
• dc.subject.other Cèl·lules
• dc.title Yeast-to-hypha transition of schizosaccharomyces japonicus in response to environmental stimuli
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion