Gastruloid development competence discriminates different states of pluripotency
Full item page Simple item page
- dc.contributor.author Cermola, Federica
- dc.contributor.author D'Aniello, Cristina
- dc.contributor.author Tatè, Rosarita
- dc.contributor.author de Cesare, Dario
- dc.contributor.author Martínez Arias, Alfonso
- dc.contributor.author Minchiotti, Gabriella
- dc.contributor.author Patriarca, Eduardo Jorge
- dc.date.accessioned 2022-03-18T10:53:14Z
- dc.date.available 2022-03-18T10:53:14Z
- dc.date.issued 2021
- dc.description.abstract Floating spheroidal aggregates of mouse embryonic stem cells can develop into polarized/elongated organoids, namely gastruloids. We set up a high-performing assay to measure gastruloid formation efficiency (GFE), and found that GFE decreases as pluripotency progresses from naive (GFE ≥ 95%) to primed (GFE = 0) state. Specifically, we show that primed EpiSCs fail to generate proper cell aggregates, while early-primed EpiLCs aggregate but eventually fail to develop into elongated gastruloids. Moreover, we characterized proline-induced cells (PiCs), a LIF-dependent reversible early-primed state of pluripotency, and show that PiCs are able to generate gastruloids (GFE ∼ 50%) and are also competent to differentiate into primordial germ cell-like cells. Thus, we propose the GFE assay as a valuable functional tool to discriminate different states of the pluripotency continuum.
- dc.description.sponsorship This study was supported by the Associazione Italiana per la Ricerca sul Cancro AIRC (grant IG 20736), Italian Ministry of Education-University-Research (grant CTN01_00177 Cluster ALISEI_IRMI and PRIN 2017XJ38A4), and Project SATIN-POR Campania FESR 2014/2020 to G.M. A.M-A.’s research is funded by an ERC Advanced Investigator award (834580)
- dc.format.mimetype application/pdf
- dc.identifier.citation Cermola F, Aniello D'A, Tatè R, Cesare D de, Martinez-Arias A, Minchiotti G et al. Gastruloid development competence discriminates different states of pluripotency. Stem Cell Reports. 2021 Feb 9;16(2):354-369. DOI: 10.1016/j.stemcr.2020.12.013
- dc.identifier.doi http://dx.doi.org/10.1016/j.stemcr.2020.12.013
- dc.identifier.issn 2213-6711
- dc.identifier.uri http://hdl.handle.net/10230/52719
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/834580
- dc.rights © 2020. Federica Cermola et al. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.other Embriologia
- dc.subject.other Genètica
- dc.title Gastruloid development competence discriminates different states of pluripotency
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion